Restriction map for
pF1KB3429
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BbvI BciVI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT14I
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI MwoI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PstI PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI ScaI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TseI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AclI 304 AACGTT AA^CGTT
AfaI 229 GTAC GT^AC
AluI 455 AGCT AG^CT
ApaI 84 GGGCCC GGGCC^C
AseI 429 ATTAAT AT^TAAT
AsnI 429 ATTAAT AT^TAAT
BanII 84 GGGCCC G[GA]GC[CT]^C
BbsI 3 GAAGAC GAAGAC(2/6)
BcgI 68 GCACAGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 239 TGATCA T^GATCA
BcnI 165 CCGGG CC^[GC]GG
BmyI 84 GGGCCC G[AGT]GC[ACT]^C
BpuAI 3 GAAGAC GAAGAC(2/6)
Bsp1286I 84 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 228 TGTACA T^GTACA
BsrGI 228 TGTACA T^GTACA
BstXI 315 CCAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 240 GATC ^GATC
EcoRV 511 GATATC GAT^ATC
FbaI 239 TGATCA T^GATCA
Fnu4HI 472 GCGGC GC^[ACGT]GC
HaeIII 85 GGCC GG^CC
HapII 165 CCGG C^CGG
HgaI 65 GACGC GACGC(5/10)
HinfI 480 GATTC G^A[ACGT]TC
HpaII 165 CCGG C^CGG
HphI 355 GGTGA GGTGA(8/7)
ItaI 472 GCGGC GC^[ACGT]GC
MaeII 305 ACGT A^CGT
MboI 240 GATC ^GATC
MboII 3 GAAGA GAAGA(8/7)
MslI 118 CACATCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 470 CAGCGG C[AC]G^C[GT]G
MspI 165 CCGG C^CGG
NciI 165 CCGGG CC^[GC]GG
NdeII 240 GATC ^GATC
PshBI 429 ATTAAT AT^TAAT
Psp1406I 304 AACGTT AA^CGTT
RsaI 229 GTAC GT^AC
Sau3AI 240 GATC ^GATC
SexAI 387 ACCAGGT A^CC[AT]GGT
SmlI 410 CTCAAG C^T[CT][GA]AG
SspBI 228 TGTACA T^GTACA
TfiI 480 GATTC G^A[AT]TC
TspRI 254 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 28 GTCGAC GT^[AC][GT]AC
46 GTCGAC
BsaI 384 GAGACC GGTCTC(1/5)
326 GGTCTC
BsiYI 98 CCCTGCGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
99 CCTGCGGAGGG
BslI 98 CCCTGCGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
99 CCTGCGGAGGG
BsmFI 63 GGGAC GGGAC(10/14)
107 GGGAC
BsrI 198 ACTGG ACTGG(1/-1)
255 ACTGG
BstNI 315 CCAGG CC^[AT]GG
388 CCAGG
Cac8I 486 GCAAGC GC[ACGT]^[ACGT]GC
564 GCAGGC
Cfr13I 84 GGGCC G^G[ACGT]CC
85 GGCCC
DdeI 13 CTCAG C^T[ACGT]AG
133 CTCAG
DraII 83 AGGGCCC [GA]G^G[ACGT]CC[CT]
84 GGGCCCC
EcoO109I 83 AGGGCCC [GA]G^G[ACGT]CC[CT]
84 GGGCCCC
EcoRII 315 CCAGG ^CC[AT]GG
388 CCAGG
MseI 430 TTAA T^TAA
500 TTAA
MvaI 315 CCAGG CC^[AT]GG
388 CCAGG
NlaIII 226 CATG CATG^
237 CATG
SalI 28 GTCGAC G^TCGAC
46 GTCGAC
Sau96I 84 GGGCC G^G[ACGT]CC
85 GGCCC
TaqI 29 TCGA T^CGA
47 TCGA
Tru9I 430 TTAA T^TAA
500 TTAA
TthHB8I 29 TCGA T^CGA
47 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI AcsI ApoI BsmAI BssKI
BstF5I FokI HincII HindII MaeIII
MnlI NlaIV ScrFI Tsp509I