##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BbeI        BbsI        BbvCI       BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeIII      MamI        MboII       MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        TaqI        TfiI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

ApaI          239  GGGCCC               GGGCC^C
Asp700I       262  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII           3  GGTCC                G^G[AT]CC
BamHI         215  GGATCC               G^GATCC
BbrPI         344  CACGTG               CAC^GTG
BclI          308  TGATCA               T^GATCA
BfaI          208  CTAG                 C^TAG
BsaAI         344  CACGTG               [CT]AC^GT[GA]
BseRI          19  CTCCTC               GAGGAG(10/8)
BsgI           75  CTGCAC               GTGCAG(16/14)
BsiEI          85  CGGCCG               CG[GA][CT]^CG
BsmAI         324  GAGAC                GTCTC(1/5)
BsmFI         122  GTCCC                GGGAC(10/14)
BsrDI         167  CATTGC               GCAATG(2/0)
BstYI         215  GGATCC               [GA]^GATC[CT]
Cac8I         280  GCTGGC               GC[ACGT]^[ACGT]GC
DraII         238  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DsaI           36  CCGTGG               C^C[GA][CT]GG
EagI           85  CGGCCG               C^GGCCG
EclXI          85  CGGCCG               C^GGCCG
Eco52I         85  CGGCCG               C^GGCCG
EcoNI          21  CCTCTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      238  GGGGCCC              [GA]G^G[ACGT]CC[CT]
FauI          236  GCGGG                CCCGC(4/6)
FbaI          308  TGATCA               T^GATCA
HindIII       265  AAGCTT               A^AGCTT
MaeI          208  CTAG                 C^TAG
MaeII         345  ACGT                 A^CGT
MflI          215  GGATCC               [GA]^GATC[CT]
MseI           49  TTAA                 T^TAA
MspA1I        148  CAGCTG               C[AC]G^C[GT]G
NlaIII        181  CATG                 CATG^
PmaCI         344  CACGTG               CAC^GTG
PmlI          344  CACGTG               CAC^GTG
PvuII         148  CAGCTG               CAG^CTG
StuI           29  AGGCCT               AGG^CCT
Tru9I          49  TTAA                 T^TAA
XhoII         215  GGATCC               [GA]^GATC[CT]
XmnI          262  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BanII         153  GGGCTC               G[GA]GC[CT]^C
              239  GGGCCC              
BssKI          66  CCTGG                ^CC[ACGT]GG
              125  CCAGG               
BstNI          66  CCTGG                CC^[AT]GG
              125  CCAGG               
EaeI           85  CGGCCG               [CT]^GGCC[GA]
              132  TGGCCG              
EcoRII         66  CCTGG                ^CC[AT]GG
              125  CCAGG               
MvaI           66  CCTGG                CC^[AT]GG
              125  CCAGG               
ScrFI          66  CCTGG                CC^[ACGT]GG
              125  CCAGG               
SfaNI         376  GATGC                GCATC(5/9)
              297  GCATC               
SfcI          386  CTACAG               C^T[GA][CT]AG
              401  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BstF5I      
Cfr13I      DdeI        DpnII       Fnu4HI      FokI        
HaeIII      ItaI        MboI        MnlI        NdeII       
NlaIV       Sau3AI      Sau96I      TseI