Restriction map for
pF1KB3401
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlpI Bpu1102I
BpuAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MboI MfeI MflI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NdeII
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 546 ACGTGT A^C[GA][CT]GT
AlwNI 23 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 399 GGGCCC GGGCC^C
AvrII 505 CCTAGG C^CTAGG
BanII 399 GGGCCC G[GA]GC[CT]^C
BbrPI 545 CACGTG CAC^GTG
BglI 392 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 505 CCTAGG C^CTAGG
BmyI 399 GGGCCC G[AGT]GC[ACT]^C
BpmI 266 CTGGAG CTGGAG(16/14)
BsaAI 545 CACGTG [CT]AC^GT[GA]
BsaBI 307 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 22 CCAGCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 22 CCAGCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 690 GAGAC GTCTC(1/5)
BsmBI 690 GAGACG CGTCTC(1/5)
BsmI 240 GCATTC GAATGC(1/-1)
Bsp1286I 399 GGGCCC G[AGT]GC[ACT]^C
BstAPI 619 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 306 GGATG GGATG(2/0)
CfoI 669 GCGC GCG^C
DraII 399 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 207 CCATGG C^C[GA][CT]GG
EcoO109I 399 GGGCCCC [GA]G^G[ACGT]CC[CT]
FokI 306 GGATG GGATG(9/13)
HaeII 668 AGCGCC [GA]GCGC^[CT]
HhaI 669 GCGC GCG^C
HinP1I 669 GCGC G^CGC
HphI 124 GGTGA GGTGA(8/7)
MaeII 546 ACGT A^CGT
MamI 307 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 484 TTAA T^TAA
NcoI 207 CCATGG C^CATGG
NheI 412 GCTAGC G^CTAGC
PflMI 22 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 545 CACGTG CAC^GTG
PmlI 545 CACGTG CAC^GTG
SfaNI 307 GATGC GCATC(5/9)
Tru9I 484 TTAA T^TAA
Tsp509I 681 AATT ^AATT
Van91I 22 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 74 GTAC GT^AC
593 GTAC
AvaII 147 GGTCC G^G[AT]CC
509 GGACC
BalI 95 TGGCCA TGG^CCA
638 TGGCCA
BfaI 413 CTAG C^TAG
506 CTAG
EarI 610 GAAGAG CTCTTC(1/4)
687 GAAGAG
Ksp632I 610 GAAGAG CTCTTC(1/4)
687 GAAGAG
MaeI 413 CTAG C^TAG
506 CTAG
MaeIII 248 GTTAC ^GT[ACGT]AC
538 GTTAC
MluNI 95 TGGCCA TGG^CCA
638 TGGCCA
MscI 95 TGGCCA TGG^CCA
638 TGGCCA
MslI 45 CATTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
208 CATGGCCGTG
NlaIII 186 CATG CATG^
208 CATG
NlaIV 399 GGGCCC GG[ACGT]^[ACGT]CC
400 GGCCCC
PstI 553 CTGCAG CTGCA^G
617 CTGCAG
RsaI 74 GTAC GT^AC
593 GTAC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
EaeI EcoRII EcoT14I Fnu4HI HaeIII
HgaI HinfI ItaI MboII MnlI
MvaI MwoI Sau96I ScrFI SfcI
StyI TfiI TseI TspRI