Restriction map for
pF1KB3388
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI DdeI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AcyI 416 GGCGCC G[GA]^CG[CT]C
AhdI 599 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 518 AGCT AG^CT
AlwI 495 GATCC GGATC(4/5)
ApaI 221 GGGCCC GGGCC^C
AspEI 599 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 200 CCCGAG C^[CT]CG[GA]G
BanI 416 GGCGCC G^G[CT][GA]CC
BanII 221 GGGCCC G[GA]GC[CT]^C
BbeI 416 GGCGCC GGCGC^C
BcnI 219 CCGGG CC^[GC]GG
BglI 61 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 221 GGGCCC G[AGT]GC[ACT]^C
BpmI 466 CTGGAG CTGGAG(16/14)
BsaHI 416 GGCGCC G[GA]^CG[CT]C
BsaWI 503 ACCGGA [AT]^CCGG[AT]
BseRI 469 GAGGAG GAGGAG(10/8)
BsgI 10 GTGCAG GTGCAG(16/14)
BsiWI 105 CGTACG C^GTACG
BsmAI 302 GTCTC GTCTC(1/5)
BsmBI 301 CGTCTC CGTCTC(1/5)
BsoBI 200 CCCGAG C^[CT]CG[GA]G
Bsp1286I 221 GGGCCC G[AGT]GC[ACT]^C
BspHI 521 TCATGA T^CATGA
BspMI 12 GCAGGT ACCTGC(4/8)
BsrBI 290 CCGCTC CCGCTC(-3/-3)
BssSI 427 CACGAG CACGAG(-5/-1)
BstYI 494 AGATCC [GA]^GATC[CT]
Cac8I 176 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 417 GCGC GCG^C
CpoI 244 CGGACCG CG^G[AT]CCG
DpnII 495 GATC ^GATC
DsaI 594 CCACGG C^C[GA][CT]GG
EaeI 59 CGGCCA [CT]^GGCC[GA]
Eam1105I 599 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 474 GAAGAG CTCTTC(1/4)
EcoNI 196 CCTTCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 416 GGCGCC [GA]GCGC^[CT]
HgaI 381 GCGTC GACGC(5/10)
HhaI 417 GCGC GCG^C
Hin1I 416 GGCGCC G[GA]^CG[CT]C
HinP1I 417 GCGC G^CGC
HinfI 599 GACTC G^A[ACGT]TC
KasI 416 GGCGCC G^GCGCC
Ksp632I 474 GAAGAG CTCTTC(1/4)
MaeII 551 ACGT A^CGT
MaeIII 454 GTGAC ^GT[ACGT]AC
MboI 495 GATC ^GATC
MflI 494 AGATCC [GA]^GATC[CT]
NarI 416 GGCGCC GG^CGCC
NciI 219 CCGGG CC^[GC]GG
NdeII 495 GATC ^GATC
NlaIII 522 CATG CATG^
PflMI 39 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 599 GACTC GAGTC(4/5)
RcaI 521 TCATGA T^CATGA
RsrII 244 CGGACCG CG^G[AT]CCG
Sau3AI 495 GATC ^GATC
SfcI 141 CTACAG C^T[GA][CT]AG
SfoI 416 GGCGCC GGC^GCC
TaqI 33 TCGA T^CGA
Tsp45I 454 GTGAC ^GT[GC]AC
TspRI 452 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 33 TCGA T^CGA
Van91I 39 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 494 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 5 enzymes) #
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AlwNI 13 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
76 CAGGTACTG
BbvI 261 GCTGC GCAGC(8/12)
364 GCTGC
PpuMI 14 AGGTCCT [GA]G^G[AT]CC[CT]
581 AGGTCCT
TseI 261 GCTGC G^C[AT]GC
364 GCTGC
Tsp509I 136 AATT ^AATT
564 AATT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AvaII BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MboII
MnlI MspI MvaI MwoI NlaIV
RsaI Sau96I ScrFI SfaNI SmlI