Restriction map for
pF1KB3113
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BfaI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HaeII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 681 CTTAAG C^TTAAG
AflIII 240 ACATGT A^C[GA][CT]GT
Alw44I 444 GTGCAC G^TGCAC
AlwNI 532 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 521 CCTCAGG CC^T[ACGT]AGG
ApaLI 444 GTGCAC G^TGCAC
AspHI 444 GTGCAC G[AT]GC[AT]^C
BanII 891 GAGCCC G[GA]GC[CT]^C
BbsI 884 GTCTTC GAAGAC(2/6)
BclI 528 TGATCA T^GATCA
BfrI 681 CTTAAG C^TTAAG
BglII 935 AGATCT A^GATCT
BpuAI 884 GTCTTC GAAGAC(2/6)
BsaI 488 GGTCTC GGTCTC(1/5)
BsgI 584 CTGCAC GTGCAG(16/14)
BsiHKAI 444 GTGCAC G[AT]GC[AT]^C
BspLU11I 240 ACATGT A^CATGT
BspMI 80 ACCTGC ACCTGC(4/8)
BsrBI 20 GAGCGG CCGCTC(-3/-3)
BstEII 642 GGTGACC G^GT[ACGT]ACC
BstPI 642 GGTGACC G^GT[ACGT]ACC
Bsu36I 521 CCTCAGG CC^T[ACGT]AGG
DsaI 51 CCACGG C^C[GA][CT]GG
EarI 830 CTCTTC CTCTTC(1/4)
Eco81I 521 CCTCAGG CC^T[ACGT]AGG
EcoO65I 642 GGTGACC G^GT[ACGT]ACC
FauI 127 GCGGG CCCGC(4/6)
FbaI 528 TGATCA T^GATCA
HincII 655 GTTGAC GT[CT]^[GA]AC
HindII 655 GTTGAC GT[CT]^[GA]AC
HindIII 186 AAGCTT A^AGCTT
Ksp632I 830 CTCTTC CTCTTC(1/4)
MseI 682 TTAA T^TAA
NspI 240 ACATGT [GA]CATG^[CT]
PflMI 424 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 877 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SspI 147 AATATT AAT^ATT
Tru9I 682 TTAA T^TAA
Van91I 424 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 633 GAATTT [GA]^AATT[CT]
745 AAATTT
AcyI 39 GACGCC G[GA]^CG[CT]C
47 GACGCC
AfaI 78 GTAC GT^AC
691 GTAC
ApoI 633 GAATTT [GA]^AATT[CT]
745 AAATTT
BamHI 153 GGATCC G^GATCC
420 GGATCC
BmyI 444 GTGCAC G[AGT]GC[ACT]^C
891 GAGCCC
BsaHI 39 GACGCC G[GA]^CG[CT]C
47 GACGCC
BsiYI 424 CCAACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
894 CCCCACTCAGG
BslI 424 CCAACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
894 CCCCACTCAGG
BsmAI 438 GTCTC GTCTC(1/5)
489 GTCTC
Bsp1286I 444 GTGCAC G[AGT]GC[ACT]^C
891 GAGCCC
BstNI 93 CCTGG CC^[AT]GG
502 CCTGG
BstXI 495 CCACATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
896 CCACTCAGGTGG
Cfr13I 87 GGGCC G^G[ACGT]CC
304 GGGCC
EcoRII 93 CCTGG ^CC[AT]GG
502 CCTGG
HgaI 39 GACGC GACGC(5/10)
47 GACGC
Hin1I 39 GACGCC G[GA]^CG[CT]C
47 GACGCC
MboII 831 TCTTC GAAGA(8/7)
885 TCTTC
MslI 721 CATACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
897 CACTCAGGTG
MvaI 93 CCTGG CC^[AT]GG
502 CCTGG
PleI 534 GACTC GAGTC(4/5)
826 GACTC
PstI 274 CTGCAG CTGCA^G
299 CTGCAG
RsaI 78 GTAC GT^AC
691 GTAC
Sau96I 87 GGGCC G^G[ACGT]CC
304 GGGCC
SmlI 603 CTTGAG C^T[CT][GA]AG
681 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BcnI BsaJI BsmFI BsrI BssKI
BstF5I BstUI BstYI Cac8I CfoI
DdeI DpnII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MflI
MnlI MspI MvnI MwoI NciI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfaNI SfcI TfiI TseI Tsp45I
Tsp509I TspRI XhoII