Restriction map for
pF1KB3050
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SphI SspBI SspI SwaI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 160 CGCG CG^CG
AcsI 403 AAATTT [GA]^AATT[CT]
ApoI 403 AAATTT [GA]^AATT[CT]
AspI 1420 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 165 GGATCC G^GATCC
BbvCI 118 GCTGAGG CCTCAGC(-5/-2)
BlpI 681 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 681 GCTGAGC GC^T[ACGT]AGC
BsrDI 1428 CATTGC GCAATG(2/0)
BsrFI 77 GCCGGC [GA]^CCGG[CT]
BstUI 160 CGCG CG^CG
BstYI 165 GGATCC [GA]^GATC[CT]
CelII 681 GCTGAGC GC^T[ACGT]AGC
Cfr10I 77 GCCGGC [GA]^CCGG[CT]
DraIII 445 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1398 GAAGAG CTCTTC(1/4)
HaeII 723 GGCGCT [GA]GCGC^[CT]
Ksp632I 1398 GAAGAG CTCTTC(1/4)
MaeIII 547 GTGAC ^GT[ACGT]AC
MflI 165 GGATCC [GA]^GATC[CT]
MvnI 160 CGCG CG^CG
NaeI 77 GCCGGC GCC^GGC
NgoMIV 77 GCCGGC G^CCGGC
PflFI 1420 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1009 GACTC GAGTC(4/5)
SapI 1398 GAAGAGC GCTCTTC(1/4)
SbfI 912 CCTGCAGG CCTGCA^GG
SpeI 1207 ACTAGT A^CTAGT
Sse8387I 912 CCTGCAGG CCTGCA^GG
StuI 713 AGGCCT AGG^CCT
TfiI 1127 GATTC G^A[AT]TC
Tsp45I 547 GTGAC ^GT[GC]AC
Tth111I 1420 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 165 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 172 ACATGT A^C[GA][CT]GT
1334 ACATGT
AlwNI 907 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
920 CAGTCTCTG
ApaI 57 GGGCCC GGGCC^C
1316 GGGCCC
AspHI 1016 GAGCAC G[AT]GC[AT]^C
1366 GAGCAC
BbsI 936 GAAGAC GAAGAC(2/6)
1120 GAAGAC
BpuAI 936 GAAGAC GAAGAC(2/6)
1120 GAAGAC
BsgI 222 CTGCAC GTGCAG(16/14)
559 GTGCAG
BsiHKAI 1016 GAGCAC G[AT]GC[AT]^C
1366 GAGCAC
BspLU11I 172 ACATGT A^CATGT
1334 ACATGT
BspMI 307 ACCTGC ACCTGC(4/8)
1601 ACCTGC
BsrBI 108 CCGCTC CCGCTC(-3/-3)
436 GAGCGG
EcoNI 1341 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1602 CCTGCTGGAGG
HinfI 1009 GACTC G^A[ACGT]TC
1127 GATTC
NcoI 20 CCATGG C^CATGG
635 CCATGG
PpuMI 98 AGGACCC [GA]G^G[AT]CC[CT]
196 GGGACCC
SfiI 123 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1185 GGCCGTGGTGGCC
SmaI 193 CCCGGG CCC^GGG
459 CCCGGG
SmlI 127 CTCAAG C^T[CT][GA]AG
985 CTCAAG
Tsp509I 404 AATT ^AATT
841 AATT
XcmI 635 CCATGGCCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1178 CCAACATGGCCGTGG
XmaCI 193 CCCGGG C^CCGGG
459 CCCGGG
XmaI 193 CCCGGG C^CCGGG
459 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeI MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I Van91I