Restriction map for
pF1KE0983
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AcyI AflIII
AgeI Alw44I AlwNI AocI ApaI
ApaLI AscI Asp718I AspI AvrII
BamHI BbeI BbrPI BcgI BciVI
BglI BglII BlnI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrBI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstUI Bsu36I Cfr10I CpoI DraI
DraIII DrdI EagI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PstI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 1727 GTATAC GT^[AC][GT]AC
AclI 808 AACGTT AA^CGTT
AflII 778 CTTAAG C^TTAAG
AlwI 759 GATCC GGATC(4/5)
Aor51HI 731 AGCGCT AGC^GCT
Asp700I 1015 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1127 GAGCTC G[AT]GC[AT]^C
AvaI 602 CTCGAG C^[CT]CG[GA]G
AviII 719 TGCGCA TGC^GCA
BalI 668 TGGCCA TGG^CCA
BbvCI 751 GCTGAGG CCTCAGC(-5/-2)
BclI 681 TGATCA T^GATCA
BcnI 53 CCGGG CC^[GC]GG
BfrI 778 CTTAAG C^TTAAG
BlpI 870 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 870 GCTGAGC GC^T[ACGT]AGC
BseRI 754 GAGGAG GAGGAG(10/8)
BsiHKAI 1127 GAGCTC G[AT]GC[AT]^C
BsoBI 602 CTCGAG C^[CT]CG[GA]G
BspDI 620 ATCGAT AT^CGAT
BspHI 104 TCATGA T^CATGA
BspMI 663 GCAGGT ACCTGC(4/8)
BsrDI 722 GCAATG GCAATG(2/0)
BssSI 14 CTCGTG CACGAG(-5/-1)
Bst1107I 1727 GTATAC GTA^TAC
BstXI 1368 CCAGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 758 AGATCC [GA]^GATC[CT]
BstZ17I 1727 GTATAC GTA^TAC
CelII 870 GCTGAGC GC^T[ACGT]AGC
ClaI 620 ATCGAT AT^CGAT
DsaI 1035 CCACGG C^C[GA][CT]GG
EaeI 668 TGGCCA [CT]^GGCC[GA]
EarI 1222 CTCTTC CTCTTC(1/4)
Eco47III 731 AGCGCT AGC^GCT
FbaI 681 TGATCA T^GATCA
FspI 719 TGCGCA TGC^GCA
HaeII 731 AGCGCT [GA]GCGC^[CT]
HgaI 923 GCGTC GACGC(5/10)
HindIII 1690 AAGCTT A^AGCTT
Ksp632I 1222 CTCTTC CTCTTC(1/4)
MfeI 1166 CAATTG C^AATTG
MflI 758 AGATCC [GA]^GATC[CT]
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MslI 99 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1166 CAATTG C^AATTG
NciI 53 CCGGG CC^[GC]GG
NdeI 1173 CATATG CA^TATG
NspI 818 ACATGC [GA]CATG^[CT]
PaeR7I 602 CTCGAG C^TCGAG
Psp1406I 808 AACGTT AA^CGTT
RcaI 104 TCATGA T^CATGA
SacI 1127 GAGCTC GAGCT^C
ScaI 1319 AGTACT AGT^ACT
XcmI 1165 CCAATTGACATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 602 CTCGAG C^TCGAG
XhoII 758 AGATCC [GA]^GATC[CT]
XmnI 1015 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 761 TCCGGA T^CCGGA
839 TCCGGA
AcsI 271 GAATTT [GA]^AATT[CT]
500 AAATTT
AhdI 1063 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1600 GACAAGGTGTC
ApoI 271 GAATTT [GA]^AATT[CT]
500 AAATTT
AseI 487 ATTAAT AT^TAAT
1327 ATTAAT
AsnI 487 ATTAAT AT^TAAT
1327 ATTAAT
AspEI 1063 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1600 GACAAGGTGTC
BanI 402 GGCACC G^G[CT][GA]CC
414 GGTGCC
BanII 55 GGGCTC G[GA]GC[CT]^C
1127 GAGCTC
BbsI 179 GAAGAC GAAGAC(2/6)
1505 GTCTTC
BfaI 613 CTAG C^TAG
1273 CTAG
BmyI 55 GGGCTC G[AGT]GC[ACT]^C
1127 GAGCTC
BpuAI 179 GAAGAC GAAGAC(2/6)
1505 GTCTTC
BsaAI 700 TACGTG [CT]AC^GT[GA]
1406 CACGTA
BsaI 605 GAGACC GGTCTC(1/5)
1244 GAGACC
BsaWI 761 TCCGGA [AT]^CCGG[AT]
839 TCCGGA
BseAI 761 TCCGGA T^CCGGA
839 TCCGGA
Bsp1286I 55 GGGCTC G[AGT]GC[ACT]^C
1127 GAGCTC
BspEI 761 TCCGGA T^CCGGA
839 TCCGGA
Cac8I 1255 GCCTGC GC[ACGT]^[ACGT]GC
1525 GCCCGC
Eam1105I 1063 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1600 GACAAGGTGTC
HinfI 319 GACTC G^A[ACGT]TC
423 GACTC
HphI 241 GGTGA GGTGA(8/7)
1589 TCACC
MaeI 613 CTAG C^TAG
1273 CTAG
MaeIII 317 GTGAC ^GT[ACGT]AC
1360 GTGAC
MroI 761 TCCGGA T^CCGGA
839 TCCGGA
PleI 319 GACTC GAGTC(4/5)
423 GACTC
PpuMI 1425 GGGTCCC [GA]G^G[AT]CC[CT]
1514 GGGACCC
PshBI 487 ATTAAT AT^TAAT
1327 ATTAAT
SfcI 1122 CTACAG C^T[GA][CT]AG
1155 CTACAG
Tsp45I 317 GTGAC ^GT[GC]AC
1360 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I