Restriction map for
pF1KE0963
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmFI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FauI FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII ItaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspI PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
TseI Tth111I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 363 ACGTGT A^C[GA][CT]GT
AseI 925 ATTAAT AT^TAAT
AsnI 925 ATTAAT AT^TAAT
Asp700I 393 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 50 GAGCTC G[AT]GC[AT]^C
BamHI 1344 GGATCC G^GATCC
BsaI 1177 GAGACC GGTCTC(1/5)
BsiHKAI 50 GAGCTC G[AT]GC[AT]^C
BsiYI 1410 CCCCGACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1410 CCCCGACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 668 CGTCTC CGTCTC(1/5)
BsmI 1209 GAATGC GAATGC(1/-1)
BspMI 527 ACCTGC ACCTGC(4/8)
BstBI 849 TTCGAA TT^CGAA
Cac8I 1437 GCGAGC GC[ACGT]^[ACGT]GC
DraI 712 TTTAAA TTT^AAA
DraII 1278 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 7 GACTACATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 956 GAAGAG CTCTTC(1/4)
EcoO109I 1278 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 135 GAATTC G^AATTC
EcoRV 379 GATATC GAT^ATC
EcoT22I 1079 ATGCAT ATGCA^T
Ksp632I 956 GAAGAG CTCTTC(1/4)
MaeII 363 ACGT A^CGT
NsiI 1079 ATGCAT ATGCA^T
NspV 849 TTCGAA TT^CGAA
PaeR7I 758 CTCGAG C^TCGAG
PshBI 925 ATTAAT AT^TAAT
SacI 50 GAGCTC GAGCT^C
SexAI 1254 ACCAGGT A^CC[AT]GGT
SfuI 849 TTCGAA TT^CGAA
SspI 181 AATATT AAT^ATT
StuI 1452 AGGCCT AGG^CCT
XcmI 327 CCAAATGTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 758 CTCGAG C^TCGAG
XmnI 393 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 592 CTTAAG C^TTAAG
1204 CTTAAG
AhdI 550 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1414 GACTGGGTGTC
AspEI 550 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1414 GACTGGGTGTC
AvaI 758 CTCGAG C^[CT]CG[GA]G
799 CCCGAG
AvaII 15 GGTCC G^G[AT]CC
557 GGTCC
BanII 36 GGGCTC G[GA]GC[CT]^C
50 GAGCTC
BbsI 301 GAAGAC GAAGAC(2/6)
449 GTCTTC
BfrI 592 CTTAAG C^TTAAG
1204 CTTAAG
BmyI 36 GGGCTC G[AGT]GC[ACT]^C
50 GAGCTC
BpmI 509 CTCCAG CTGGAG(16/14)
1095 CTCCAG
BpuAI 301 GAAGAC GAAGAC(2/6)
449 GTCTTC
BseRI 1375 GAGGAG GAGGAG(10/8)
1393 GAGGAG
BsoBI 758 CTCGAG C^[CT]CG[GA]G
799 CCCGAG
Bsp1286I 36 GGGCTC G[AGT]GC[ACT]^C
50 GAGCTC
BssKI 1255 CCAGG ^CC[ACGT]GG
1363 CCTGG
BstNI 1255 CCAGG CC^[AT]GG
1363 CCTGG
BstXI 458 CCAATCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
511 CCAGAAATTTGG
Eam1105I 550 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1414 GACTGGGTGTC
EcoRII 1255 CCAGG ^CC[AT]GG
1363 CCTGG
EcoT14I 336 CCTTGG C^C[AT][AT]GG
560 CCTTGG
HaeIII 1279 GGCC GG^CC
1453 GGCC
MaeIII 548 GTGAC ^GT[ACGT]AC
1325 GTCAC
MvaI 1255 CCAGG CC^[AT]GG
1363 CCTGG
ScrFI 1255 CCAGG CC^[ACGT]GG
1363 CCTGG
StyI 336 CCTTGG C^C[AT][AT]GG
560 CCTTGG
TaqI 759 TCGA T^CGA
850 TCGA
Tsp45I 548 GTGAC ^GT[GC]AC
1325 GTCAC
TthHB8I 759 TCGA T^CGA
850 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BsaJI BsmAI BsrI BstF5I
BstYI Cfr13I DdeI DpnII FokI
HinfI HphI MaeI MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
SfaNI SmlI TfiI Tru9I Tsp509I
TspRI XhoII