Restriction map for
pF1KE0959
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I ClaI CpoI DpnII
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MaeII MamI MboI MfeI
MflI MluI MroI MseI MunI
NarI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AccII 1085 CGCG CG^CG
AvrII 1305 CCTAGG C^CTAGG
BalI 23 TGGCCA TGG^CCA
BbvCI 1464 CCTCAGC CCTCAGC(-5/-2)
BlnI 1305 CCTAGG C^CTAGG
BsaI 806 GAGACC GGTCTC(1/5)
BsmI 179 GCATTC GAATGC(1/-1)
BspHI 1010 TCATGA T^CATGA
BssSI 481 CACGAG CACGAG(-5/-1)
BstUI 1085 CGCG CG^CG
EarI 196 CTCTTC CTCTTC(1/4)
Ksp632I 196 CTCTTC CTCTTC(1/4)
KspI 1084 CCGCGG CCGC^GG
MluNI 23 TGGCCA TGG^CCA
MscI 23 TGGCCA TGG^CCA
MvnI 1085 CGCG CG^CG
NcoI 351 CCATGG C^CATGG
NspI 898 GCATGC [GA]CATG^[CT]
RcaI 1010 TCATGA T^CATGA
SacII 1084 CCGCGG CCGC^GG
SmaI 728 CCCGGG CCC^GGG
SmlI 121 CTCAAG C^T[CT][GA]AG
SphI 898 GCATGC GCATG^C
TfiI 113 GATTC G^A[AT]TC
XmaCI 728 CCCGGG C^CCGGG
XmaI 728 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 549 GTAC GT^AC
776 GTAC
Alw44I 688 GTGCAC G^TGCAC
1377 GTGCAC
AocI 616 CCTGAGG CC^T[ACGT]AGG
1324 CCTGAGG
ApaLI 688 GTGCAC G^TGCAC
1377 GTGCAC
BbsI 370 GTCTTC GAAGAC(2/6)
984 GTCTTC
BlpI 138 GCTCAGC GC^T[ACGT]AGC
336 GCTCAGC
Bpu1102I 138 GCTCAGC GC^T[ACGT]AGC
336 GCTCAGC
BpuAI 370 GTCTTC GAAGAC(2/6)
984 GTCTTC
BsgI 17 CTGCAC GTGCAG(16/14)
670 CTGCAC
BsiEI 396 CGGCCG CG[GA][CT]^CG
1046 CGGCCG
BsmAI 268 GAGAC GTCTC(1/5)
806 GAGAC
Bsu36I 616 CCTGAGG CC^T[ACGT]AGG
1324 CCTGAGG
CelII 138 GCTCAGC GC^T[ACGT]AGC
336 GCTCAGC
CfoI 381 GCGC GCG^C
945 GCGC
EagI 396 CGGCCG C^GGCCG
1046 CGGCCG
EclXI 396 CGGCCG C^GGCCG
1046 CGGCCG
Eco52I 396 CGGCCG C^GGCCG
1046 CGGCCG
Eco81I 616 CCTGAGG CC^T[ACGT]AGG
1324 CCTGAGG
HhaI 381 GCGC GCG^C
945 GCGC
HinP1I 381 GCGC G^CGC
945 GCGC
MslI 27 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
691 CACCTTGATG
NaeI 299 GCCGGC GCC^GGC
398 GCCGGC
NgoMIV 299 GCCGGC G^CCGGC
398 GCCGGC
PflMI 678 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCAGCAACTGG
PleI 270 GACTC GAGTC(4/5)
307 GAGTC
RsaI 549 GTAC GT^AC
776 GTAC
Van91I 678 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCAGCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PstI PvuII Sau96I ScrFI
SfaNI SfcI StuI StyI TseI
Tsp45I TspRI XcmI