Restriction map for
pF1KE0952
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BbeI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MseI MunI MvnI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AocI 346 CCTCAGG CC^T[ACGT]AGG
BalI 716 TGGCCA TGG^CCA
BamHI 942 GGATCC G^GATCC
BanI 1416 GGTGCC G^G[CT][GA]CC
BbrPI 1015 CACGTG CAC^GTG
BbvCI 1344 GCTGAGG CCTCAGC(-5/-2)
BciVI 32 GTATCC GTATCC(6/5)
BfaI 1357 CTAG C^TAG
BglI 1001 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1276 GAGACC GGTCTC(1/5)
BspHI 1199 TCATGA T^CATGA
BspLU11I 120 ACATGT A^CATGT
BsrFI 863 GCCGGC [GA]^CCGG[CT]
BssSI 1048 CACGAG CACGAG(-5/-1)
BstAPI 1371 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 351 GGTGACC G^GT[ACGT]ACC
BstPI 351 GGTGACC G^GT[ACGT]ACC
Bsu36I 346 CCTCAGG CC^T[ACGT]AGG
CfoI 627 GCGC GCG^C
Cfr10I 863 GCCGGC [GA]^CCGG[CT]
DrdI 1247 GACCCTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1165 CCATGG C^C[GA][CT]GG
EaeI 716 TGGCCA [CT]^GGCC[GA]
Eco81I 346 CCTCAGG CC^T[ACGT]AGG
EcoNI 1080 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 351 GGTGACC G^GT[ACGT]ACC
HaeII 626 GGCGCT [GA]GCGC^[CT]
HgaI 414 GACGC GACGC(5/10)
HhaI 627 GCGC GCG^C
HinP1I 627 GCGC G^CGC
HincII 679 GTCAAC GT[CT]^[GA]AC
HindII 679 GTCAAC GT[CT]^[GA]AC
MaeI 1357 CTAG C^TAG
MluNI 716 TGGCCA TGG^CCA
MscI 716 TGGCCA TGG^CCA
NaeI 863 GCCGGC GCC^GGC
NcoI 1165 CCATGG C^CATGG
NdeI 1391 CATATG CA^TATG
NgoMIV 863 GCCGGC G^CCGGC
PmaCI 1015 CACGTG CAC^GTG
PmlI 1015 CACGTG CAC^GTG
RcaI 1199 TCATGA T^CATGA
SapI 339 GAAGAGC GCTCTTC(1/4)
SmlI 1060 CTTGAG C^T[CT][GA]AG
SphI 1136 GCATGC GCATG^C
TaqI 703 TCGA T^CGA
TthHB8I 703 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 49 AAATTC [GA]^AATT[CT]
69 AAATTC
AfaI 699 GTAC GT^AC
1290 GTAC
AflIII 120 ACATGT A^C[GA][CT]GT
1014 ACACGT
ApaI 1096 GGGCCC GGGCC^C
1234 GGGCCC
ApoI 49 AAATTC [GA]^AATT[CT]
69 AAATTC
Asp700I 504 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
645 GAACTTCTTC
BcgI 704 CGACCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
731 GCATGGGTTTCG
BcnI 293 CCGGG CC^[GC]GG
1231 CCGGG
BsgI 548 CTGCAC GTGCAG(16/14)
1081 CTGCAC
BsmAI 1276 GAGAC GTCTC(1/5)
1386 GTCTC
BsmI 43 GCATTC GAATGC(1/-1)
533 GCATTC
EarI 226 CTCTTC CTCTTC(1/4)
339 GAAGAG
HindIII 449 AAGCTT A^AGCTT
1361 AAGCTT
Ksp632I 226 CTCTTC CTCTTC(1/4)
339 GAAGAG
NciI 293 CCGGG CC^[GC]GG
1231 CCGGG
PflMI 16 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1119 CCAGAGCCTGG
PshAI 105 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
952 GACCAGAGTC
PvuII 378 CAGCTG CAG^CTG
923 CAGCTG
RsaI 699 GTAC GT^AC
1290 GTAC
Van91I 16 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1119 CCAGAGCCTGG
XmnI 504 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
645 GAACTTCTTC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbsI BbvI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI XhoII