Restriction map for
pF1KE0948
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI ApoI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I CpoI
DraI DrdI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 100 GTCTAC GT^[AC][GT]AC
AccII 664 CGCG CG^CG
AfaI 288 GTAC GT^AC
AhdI 589 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1216 GTGCAC G^TGCAC
AlwNI 1066 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 863 AGCGCT AGC^GCT
ApaI 945 GGGCCC GGGCC^C
ApaLI 1216 GTGCAC G^TGCAC
AspEI 589 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 35 CTCGAG C^[CT]CG[GA]G
BlpI 302 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 302 GCTCAGC GC^T[ACGT]AGC
BsoBI 35 CTCGAG C^[CT]CG[GA]G
BspDI 112 ATCGAT AT^CGAT
BsrBI 1125 GAGCGG CCGCTC(-3/-3)
BsrDI 348 CATTGC GCAATG(2/0)
BstUI 664 CGCG CG^CG
BstXI 614 CCATCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 407 AGATCC [GA]^GATC[CT]
CelII 302 GCTCAGC GC^T[ACGT]AGC
ClaI 112 ATCGAT AT^CGAT
DraIII 1219 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 776 CCACGG C^C[GA][CT]GG
EagI 976 CGGCCG C^GGCCG
Eam1105I 589 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 55 CTCTTC CTCTTC(1/4)
EclXI 976 CGGCCG C^GGCCG
Eco47III 863 AGCGCT AGC^GCT
Eco52I 976 CGGCCG C^GGCCG
HaeII 863 AGCGCT [GA]GCGC^[CT]
HgaI 665 GCGTC GACGC(5/10)
HindIII 994 AAGCTT A^AGCTT
Ksp632I 55 CTCTTC CTCTTC(1/4)
MaeIII 455 GTGAC ^GT[ACGT]AC
MflI 407 AGATCC [GA]^GATC[CT]
MslI 519 CACATTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 664 CGCG CG^CG
PaeR7I 35 CTCGAG C^TCGAG
PflMI 266 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 288 GTAC GT^AC
SapI 54 GCTCTTC GCTCTTC(1/4)
SmlI 35 CTCGAG C^T[CT][GA]AG
StuI 743 AGGCCT AGG^CCT
Tsp45I 455 GTGAC ^GT[GC]AC
Van91I 266 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 35 CTCGAG C^TCGAG
XhoII 407 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 46 CCTGAGG CC^T[ACGT]AGG
550 CCTGAGG
Asp700I 516 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1161 GAACGCCTTC
BalI 137 TGGCCA TGG^CCA
623 TGGCCA
BanI 529 GGCACC G^G[CT][GA]CC
546 GGCACC
BbsI 825 GAAGAC GAAGAC(2/6)
1093 GTCTTC
BcnI 12 CCGGG CC^[GC]GG
1192 CCCGG
BpuAI 825 GAAGAC GAAGAC(2/6)
1093 GTCTTC
BsmAI 340 GAGAC GTCTC(1/5)
416 GAGAC
Bsu36I 46 CCTGAGG CC^T[ACGT]AGG
550 CCTGAGG
Eco81I 46 CCTGAGG CC^T[ACGT]AGG
550 CCTGAGG
EcoNI 156 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1173 CCTGGCCGAGG
EcoT14I 626 CCAAGG C^C[AT][AT]GG
782 CCAAGG
MluNI 137 TGGCCA TGG^CCA
623 TGGCCA
MscI 137 TGGCCA TGG^CCA
623 TGGCCA
MseI 320 TTAA T^TAA
509 TTAA
NciI 12 CCGGG CC^[GC]GG
1192 CCCGG
PpuMI 327 AGGTCCC [GA]G^G[AT]CC[CT]
1060 AGGTCCC
SexAI 324 ACCAGGT A^CC[AT]GGT
1220 ACCTGGT
SfaNI 110 GCATC GCATC(5/9)
1196 GCATC
StyI 626 CCAAGG C^C[AT][AT]GG
782 CCAAGG
Tru9I 320 TTAA T^TAA
509 TTAA
XmnI 516 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1161 GAACGCCTTC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI SacI Sau3AI
Sau96I ScrFI TaqI TfiI TseI
TspRI TthHB8I