Restriction map for
pF1KE0946
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AviII BamHI
BanI BbeI BbrPI BbsI BcgI
BclI BfrI BglI BlpI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BsgI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII DsaI EagI EarI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI Ksp632I KspI MfeI
MluI MroI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AhdI 913 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 801 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 940 CCTGAGG CC^T[ACGT]AGG
Aor51HI 476 AGCGCT AGC^GCT
AspEI 913 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 391 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 603 CCTAGG C^CTAGG
BbvCI 798 CCTCAGC CCTCAGC(-5/-2)
BciVI 677 GTATCC GTATCC(6/5)
BfaI 604 CTAG C^TAG
BglII 230 AGATCT A^GATCT
BlnI 603 CCTAGG C^CTAGG
BsaBI 117 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 292 CTCCTC GAGGAG(10/8)
BsiEI 129 CGACCG CG[GA][CT]^CG
BsmBI 1087 GAGACG CGTCTC(1/5)
BsmI 566 GCATTC GAATGC(1/-1)
BsrDI 994 GCAATG GCAATG(2/0)
BsrFI 773 ACCGGC [GA]^CCGG[CT]
BstEII 530 GGTCACC G^GT[ACGT]ACC
BstPI 530 GGTCACC G^GT[ACGT]ACC
BstXI 926 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 940 CCTGAGG CC^T[ACGT]AGG
Cfr10I 773 ACCGGC [GA]^CCGG[CT]
Eam1105I 913 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 476 AGCGCT AGC^GCT
Eco81I 940 CCTGAGG CC^T[ACGT]AGG
EcoO65I 530 GGTCACC G^GT[ACGT]ACC
HphI 532 TCACC GGTGA(8/7)
MaeI 604 CTAG C^TAG
MamI 117 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 666 GAAGA GAAGA(8/7)
MslI 261 CATCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 752 ACATGC [GA]CATG^[CT]
PaeR7I 295 CTCGAG C^TCGAG
PflFI 391 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 381 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 652 GACTC GAGTC(4/5)
PshAI 493 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 271 GAGCTC GAGCT^C
SalI 1044 GTCGAC G^TCGAC
ScaI 374 AGTACT AGT^ACT
TfiI 186 GATTC G^A[AT]TC
Tth111I 391 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 381 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 295 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 391 GACGTC GACGT^C
766 GACGTC
AccI 256 GTCTAC GT^[AC][GT]AC
1044 GTCGAC
AcsI 48 AAATTT [GA]^AATT[CT]
163 AAATTC
AcyI 391 GACGTC G[GA]^CG[CT]C
766 GACGTC
AfaI 375 GTAC GT^AC
588 GTAC
ApaI 1066 GGGCCC GGGCC^C
1094 GGGCCC
ApoI 48 AAATTT [GA]^AATT[CT]
163 AAATTC
AspHI 271 GAGCTC G[AT]GC[AT]^C
688 GAGCAC
BsaHI 391 GACGTC G[GA]^CG[CT]C
766 GACGTC
BsiHKAI 271 GAGCTC G[AT]GC[AT]^C
688 GAGCAC
BsmFI 404 GGGAC GGGAC(10/14)
988 GGGAC
CfoI 96 GCGC GCG^C
477 GCGC
DrdI 7 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
385 GACCTGGACGTC
EcoNI 321 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
429 CCTTGACAAGG
HaeII 95 AGCGCC [GA]GCGC^[CT]
476 AGCGCT
HhaI 96 GCGC GCG^C
477 GCGC
Hin1I 391 GACGTC G[GA]^CG[CT]C
766 GACGTC
HinP1I 96 GCGC G^CGC
477 GCGC
HinfI 186 GATTC G^A[ACGT]TC
652 GACTC
MaeII 392 ACGT A^CGT
767 ACGT
MaeIII 416 GTGAC ^GT[ACGT]AC
531 GTCAC
MwoI 477 GCGCTGCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
538 GCGTATGCGGC
PpuMI 404 GGGACCT [GA]G^G[AT]CC[CT]
737 AGGACCT
PstI 559 CTGCAG CTGCA^G
757 CTGCAG
RsaI 375 GTAC GT^AC
588 GTAC
SfaNI 669 GATGC GCATC(5/9)
813 GATGC
SfcI 559 CTGCAG C^T[GA][CT]AG
757 CTGCAG
Tsp45I 416 GTGAC ^GT[GC]AC
531 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
AvaII BalI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HpaII ItaI MboI MflI MluNI
MnlI MscI MseI MspI MvaI
MvnI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SmaI SmlI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI