Restriction map for
pF1KE0945
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AviII AvrII BamHI BanII BbeI
BbvCI BcgI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MslI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 685 GACGCC G[GA]^CG[CT]C
AfaI 504 GTAC GT^AC
AlwI 852 GATCC GGATC(4/5)
AseI 565 ATTAAT AT^TAAT
AsnI 565 ATTAAT AT^TAAT
AspHI 304 GAGCAC G[AT]GC[AT]^C
AvaI 41 CCCGAG C^[CT]CG[GA]G
BalI 542 TGGCCA TGG^CCA
BanI 48 GGTGCC G^G[CT][GA]CC
BbrPI 519 CACGTG CAC^GTG
BbsI 627 GAAGAC GAAGAC(2/6)
BciVI 1005 GGATAC GTATCC(6/5)
BclI 74 TGATCA T^GATCA
BcnI 713 CCGGG CC^[GC]GG
BfaI 460 CTAG C^TAG
BglI 584 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 627 GAAGAC GAAGAC(2/6)
BsaAI 519 CACGTG [CT]AC^GT[GA]
BsaHI 685 GACGCC G[GA]^CG[CT]C
BsaI 985 GAGACC GGTCTC(1/5)
BsgI 574 CTGCAC GTGCAG(16/14)
BsiHKAI 304 GAGCAC G[AT]GC[AT]^C
BsoBI 41 CCCGAG C^[CT]CG[GA]G
BspLU11I 396 ACATGT A^CATGT
BstYI 851 AGATCC [GA]^GATC[CT]
FbaI 74 TGATCA T^GATCA
HapII 713 CCGG C^CGG
HgaI 685 GACGC GACGC(5/10)
Hin1I 685 GACGCC G[GA]^CG[CT]C
HpaII 713 CCGG C^CGG
MaeI 460 CTAG C^TAG
MflI 851 AGATCC [GA]^GATC[CT]
MluNI 542 TGGCCA TGG^CCA
MscI 542 TGGCCA TGG^CCA
MseI 566 TTAA T^TAA
MspI 713 CCGG C^CGG
NciI 713 CCGGG CC^[GC]GG
NdeI 824 CATATG CA^TATG
PflMI 55 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 519 CACGTG CAC^GTG
PmlI 519 CACGTG CAC^GTG
PshBI 565 ATTAAT AT^TAAT
PstI 367 CTGCAG CTGCA^G
RsaI 504 GTAC GT^AC
SfcI 367 CTGCAG C^T[GA][CT]AG
SmlI 468 CTTGAG C^T[CT][GA]AG
SphI 934 GCATGC GCATG^C
StuI 30 AGGCCT AGG^CCT
TfiI 908 GATTC G^A[AT]TC
Tru9I 566 TTAA T^TAA
Tsp509I 568 AATT ^AATT
Van91I 55 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 851 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 52 CCGC CCGC(-3/-1)
731 GCGG
AflIII 396 ACATGT A^C[GA][CT]GT
518 ACACGT
AlwNI 370 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
640 CAGTATCTG
AspI 550 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
630 GACACTGTC
AvaII 150 GGACC G^G[AT]CC
903 GGTCC
BseRI 131 CTCCTC GAGGAG(10/8)
223 GAGGAG
BsiYI 39 CCCCCGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
55 CCAGCTGTTGG
BslI 39 CCCCCGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
55 CCAGCTGTTGG
BsmFI 164 GTCCC GGGAC(10/14)
636 GTCCC
BspMI 572 ACCTGC ACCTGC(4/8)
620 ACCTGC
BsrI 639 CCAGT ACTGG(1/-1)
866 CCAGT
BstF5I 426 GGATG GGATG(2/0)
716 GGATG
DsaI 360 CCATGG C^C[GA][CT]GG
545 CCATGG
EaeI 542 TGGCCA [CT]^GGCC[GA]
732 CGGCCA
FokI 426 GGATG GGATG(9/13)
716 GGATG
MspA1I 56 CAGCTG C[AC]G^C[GT]G
247 CAGCTG
NcoI 360 CCATGG C^CATGG
545 CCATGG
NspI 396 ACATGT [GA]CATG^[CT]
934 GCATGC
PflFI 550 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
630 GACACTGTC
PvuII 56 CAGCTG CAG^CTG
247 CAGCTG
TaqI 113 TCGA T^CGA
135 TCGA
Tsp45I 173 GTCAC ^GT[GC]AC
403 GTCAC
Tth111I 550 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
630 GACACTGTC
TthHB8I 113 TCGA T^CGA
135 TCGA
XcmI 766 CCAGAGCAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
866 CCAGTTTCCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI BbvI BmyI BpmI BsaJI
BsmAI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TseI TspRI