Restriction map for
pF1KE0944
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiHKAI BsiWI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FbaI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
MaeI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SpeI SphI Sse8387I SspI
StuI SwaI Tsp45I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 991 GACGTC GACGT^C
Acc65I 752 GGTACC G^GTACC
AccI 15 GTCTAC GT^[AC][GT]AC
AflIII 707 ACGTGT A^C[GA][CT]GT
AlwI 795 GATCC GGATC(4/5)
Asp718I 752 GGTACC G^GTACC
AspI 825 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 804 TGGCCA TGG^CCA
BbsI 589 GAAGAC GAAGAC(2/6)
BcnI 645 CCCGG CC^[GC]GG
BglII 665 AGATCT A^GATCT
BpmI 208 CTGGAG CTGGAG(16/14)
BpuAI 589 GAAGAC GAAGAC(2/6)
BsaAI 652 TACGTA [CT]AC^GT[GA]
BsgI 241 CTGCAC GTGCAG(16/14)
BsmBI 939 CGTCTC CGTCTC(1/5)
Bsp1407I 731 TGTACA T^GTACA
BspMI 427 ACCTGC ACCTGC(4/8)
BsrBI 883 GAGCGG CCGCTC(-3/-3)
BsrDI 166 CATTGC GCAATG(2/0)
BsrGI 731 TGTACA T^GTACA
BstAPI 952 GCATATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 70 TTCGAA TT^CGAA
BstXI 737 CCATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 140 GCCCGC GC[ACGT]^[ACGT]GC
DraII 521 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 95 CCGCGG C^C[GA][CT]GG
EaeI 804 TGGCCA [CT]^GGCC[GA]
EcoO109I 521 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 945 GAATTC G^AATTC
EcoRV 277 GATATC GAT^ATC
EcoT22I 409 ATGCAT ATGCA^T
HincII 994 GTCAAC GT[CT]^[GA]AC
HindII 994 GTCAAC GT[CT]^[GA]AC
KpnI 752 GGTACC GGTAC^C
KspI 95 CCGCGG CCGC^GG
MaeIII 332 GTAAC ^GT[ACGT]AC
MluNI 804 TGGCCA TGG^CCA
MscI 804 TGGCCA TGG^CCA
NciI 645 CCCGG CC^[GC]GG
NdeI 953 CATATG CA^TATG
NsiI 409 ATGCAT ATGCA^T
NspI 407 ACATGC [GA]CATG^[CT]
NspV 70 TTCGAA TT^CGAA
PflFI 825 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 521 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 564 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 95 CCGCGG CCGC^GG
SfcI 926 CTACAG C^T[GA][CT]AG
SfuI 70 TTCGAA TT^CGAA
SmlI 526 CTCAAG C^T[CT][GA]AG
SnaBI 652 TACGTA TAC^GTA
SspBI 731 TGTACA T^GTACA
Tth111I 825 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 50 CGCG CG^CG
96 CGCG
AcyI 592 GACGCC G[GA]^CG[CT]C
991 GACGTC
AfaI 732 GTAC GT^AC
753 GTAC
BsaHI 592 GACGCC G[GA]^CG[CT]C
991 GACGTC
BsaI 215 GAGACC GGTCTC(1/5)
367 GAGACC
BsmFI 404 GGGAC GGGAC(10/14)
521 GGGAC
BsmI 411 GCATTC GAATGC(1/-1)
860 GCATTC
BsrI 677 CCAGT ACTGG(1/-1)
807 CCAGT
BstF5I 852 CATCC GGATG(2/0)
913 CATCC
BstUI 50 CGCG CG^CG
96 CGCG
BstYI 665 AGATCT [GA]^GATC[CT]
794 AGATCC
EcoT14I 836 CCAAGG C^C[AT][AT]GG
916 CCTTGG
FokI 852 CATCC GGATG(9/13)
913 CATCC
Hin1I 592 GACGCC G[GA]^CG[CT]C
991 GACGTC
HphI 364 GGTGA GGTGA(8/7)
723 GGTGA
MflI 665 AGATCT [GA]^GATC[CT]
794 AGATCC
MseI 548 TTAA T^TAA
1025 TTAA
MslI 493 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
738 CATGTTGGTG
MvnI 50 CGCG CG^CG
96 CGCG
PvuII 282 CAGCTG CAG^CTG
979 CAGCTG
RsaI 732 GTAC GT^AC
753 GTAC
SfaNI 726 GATGC GCATC(5/9)
237 GCATC
StyI 836 CCAAGG C^C[AT][AT]GG
916 CCTTGG
TaqI 71 TCGA T^CGA
943 TCGA
TfiI 295 GAATC G^A[AT]TC
619 GATTC
Tru9I 548 TTAA T^TAA
1025 TTAA
TthHB8I 71 TCGA T^CGA
943 TCGA
XhoII 665 AGATCT [GA]^GATC[CT]
794 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaI
AvaII BanI BanII BbvI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsoBI Bsp1286I BssKI BstNI Cfr13I
DdeI DpnII EarI EcoRII FauI
Fnu4HI HaeIII HapII HgaI HinfI
HpaII ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI TseI Tsp509I
TspRI