Restriction map for
pF1KE0943
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# NO CUT ENZYMES ( 135 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseRI BsiWI
BsmAI BsmBI BsmI BspDI BspHI
BspLU11I BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 931 GACGTC GACGT^C
AccII 113 CGCG CG^CG
AccIII 284 TCCGGA T^CCGGA
AcyI 931 GACGTC G[GA]^CG[CT]C
Alw44I 127 GTGCAC G^TGCAC
AlwI 278 GGATC GGATC(4/5)
ApaLI 127 GTGCAC G^TGCAC
BfaI 136 CTAG C^TAG
BglI 434 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 209 CTCCAG CTGGAG(16/14)
BsaHI 931 GACGTC G[GA]^CG[CT]C
BseAI 284 TCCGGA T^CCGGA
BsgI 820 GTGCAG GTGCAG(16/14)
BsiEI 149 CGGCCG CG[GA][CT]^CG
Bsp1407I 2 TGTACA T^GTACA
BspEI 284 TCCGGA T^CCGGA
BsrDI 809 GCAATG GCAATG(2/0)
BsrGI 2 TGTACA T^GTACA
BssHII 112 GCGCGC G^CGCGC
BstAPI 786 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 113 CGCG CG^CG
BstYI 278 GGATCT [GA]^GATC[CT]
DpnII 279 GATC ^GATC
DraIII 309 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 149 CGGCCG C^GGCCG
EclXI 149 CGGCCG C^GGCCG
Eco52I 149 CGGCCG C^GGCCG
EcoNI 174 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 386 CCATGG C^C[AT][AT]GG
Hin1I 931 GACGTC G[GA]^CG[CT]C
MaeI 136 CTAG C^TAG
MaeII 932 ACGT A^CGT
MaeIII 479 GTGAC ^GT[ACGT]AC
MboI 279 GATC ^GATC
MflI 278 GGATCT [GA]^GATC[CT]
MroI 284 TCCGGA T^CCGGA
MvnI 113 CGCG CG^CG
NcoI 386 CCATGG C^CATGG
NdeII 279 GATC ^GATC
NspI 992 GCATGC [GA]CATG^[CT]
PvuII 541 CAGCTG CAG^CTG
SacI 405 GAGCTC GAGCT^C
Sau3AI 279 GATC ^GATC
SexAI 452 ACCTGGT A^CC[AT]GGT
SphI 992 GCATGC GCATG^C
SspBI 2 TGTACA T^GTACA
StuI 347 AGGCCT AGG^CCT
StyI 386 CCATGG C^C[AT][AT]GG
Tsp45I 479 GTGAC ^GT[GC]AC
XhoII 278 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 3 GTAC GT^AC
57 GTAC
AlwNI 298 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
374 CAGACCCTG
ApaI 490 GGGCCC GGGCC^C
608 GGGCCC
AvaI 351 CTCGGG C^[CT]CG[GA]G
493 CCCGAG
BsaWI 284 TCCGGA [AT]^CCGG[AT]
964 ACCGGA
BsoBI 351 CTCGGG C^[CT]CG[GA]G
493 CCCGAG
BsrBI 92 CCGCTC CCGCTC(-3/-3)
154 GAGCGG
BstXI 446 CCATCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
614 CCAAATGGGTGG
DsaI 386 CCATGG C^C[GA][CT]GG
945 CCACGG
HinfI 382 GACTC G^A[ACGT]TC
839 GAGTC
MslI 685 CACCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
729 CACACCTGTG
MspA1I 298 CAGCGG C[AC]G^C[GT]G
541 CAGCTG
PleI 382 GACTC GAGTC(4/5)
839 GAGTC
RsaI 3 GTAC GT^AC
57 GTAC
TspRI 313 CAGTG CA[GC]TG[ACGT][ACGT]^
834 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboII MnlI MspI MvaI MwoI
NaeI NciI NgoMIV NlaIII NlaIV
PflMI PpuMI PstI Sau96I ScrFI
SfcI TseI Van91I