Restriction map for
pF1KE0942
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BsaBI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I Cfr10I CpoI DraI DraII
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MroI MseI MunI MvnI
NaeI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tru9I Tsp509I Tth111I XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 300 ACGTGT A^C[GA][CT]GT
AvaI 343 CTCGAG C^[CT]CG[GA]G
BalI 676 TGGCCA TGG^CCA
BbeI 109 GGCGCC GGCGC^C
BbrPI 299 CACGTG CAC^GTG
BbsI 661 GAAGAC GAAGAC(2/6)
BbvCI 724 GCTGAGG CCTCAGC(-5/-2)
BciVI 635 GTATCC GTATCC(6/5)
BlpI 377 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 377 GCTCAGC GC^T[ACGT]AGC
BpuAI 661 GAAGAC GAAGAC(2/6)
BsaAI 299 CACGTG [CT]AC^GT[GA]
BsaI 541 GAGACC GGTCTC(1/5)
BsaWI 578 ACCGGA [AT]^CCGG[AT]
BsmBI 310 GAGACG CGTCTC(1/5)
BsmFI 841 GTCCC GGGAC(10/14)
BsoBI 343 CTCGAG C^[CT]CG[GA]G
BspDI 155 ATCGAT AT^CGAT
BstEII 774 GGTCACC G^GT[ACGT]ACC
BstPI 774 GGTCACC G^GT[ACGT]ACC
CelII 377 GCTCAGC GC^T[ACGT]AGC
CfoI 110 GCGC GCG^C
ClaI 155 ATCGAT AT^CGAT
DrdI 496 GACAGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 720 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 774 GGTCACC G^GT[ACGT]ACC
HaeII 109 GGCGCC [GA]GCGC^[CT]
HhaI 110 GCGC GCG^C
HinP1I 110 GCGC G^CGC
HincII 505 GTCAAC GT[CT]^[GA]AC
HindII 505 GTCAAC GT[CT]^[GA]AC
KasI 109 GGCGCC G^GCGCC
MluNI 676 TGGCCA TGG^CCA
MscI 676 TGGCCA TGG^CCA
MslI 861 CACATATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 109 GGCGCC GG^CGCC
NdeI 863 CATATG CA^TATG
PaeR7I 343 CTCGAG C^TCGAG
PflMI 192 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 299 CACGTG CAC^GTG
PmlI 299 CACGTG CAC^GTG
SfaNI 500 GCATC GCATC(5/9)
SfoI 109 GGCGCC GGC^GCC
SmlI 343 CTCGAG C^T[CT][GA]AG
StuI 872 AGGCCT AGG^CCT
Van91I 192 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 95 CCATCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 343 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 109 GGCGCC G[GA]^CG[CT]C
312 GACGCC
AlwNI 130 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
589 CAGTATCTG
AspHI 271 GAGCTC G[AT]GC[AT]^C
520 GAGCTC
AvaII 285 GGACC G^G[AT]CC
840 GGTCC
BanI 23 GGCACC G^G[CT][GA]CC
109 GGCGCC
BpmI 10 CTGGAG CTGGAG(16/14)
556 CTGGAG
BsaHI 109 GGCGCC G[GA]^CG[CT]C
312 GACGCC
BseRI 187 CTCCTC GAGGAG(10/8)
307 GAGGAG
BsgI 568 CTGCAC GTGCAG(16/14)
320 GTGCAG
BsiHKAI 271 GAGCTC G[AT]GC[AT]^C
520 GAGCTC
BsmAI 310 GAGAC GTCTC(1/5)
541 GAGAC
BsrBI 13 GAGCGG CCGCTC(-3/-3)
582 GAGCGG
DsaI 605 CCACGG C^C[GA][CT]GG
690 CCACGG
EaeI 353 CGGCCA [CT]^GGCC[GA]
676 TGGCCA
FauI 471 CCCGC CCCGC(4/6)
584 GCGGG
HapII 352 CCGG C^CGG
579 CCGG
Hin1I 109 GGCGCC G[GA]^CG[CT]C
312 GACGCC
HinfI 158 GATTC G^A[ACGT]TC
164 GAATC
HpaII 352 CCGG C^CGG
579 CCGG
HphI 365 TCACC GGTGA(8/7)
776 TCACC
MaeII 32 ACGT A^CGT
300 ACGT
MboII 661 GAAGA GAAGA(8/7)
443 TCTTC
MspI 352 CCGG C^CGG
579 CCGG
NlaIII 402 CATG CATG^
751 CATG
PstI 37 CTGCAG CTGCA^G
595 CTGCAG
SacI 271 GAGCTC GAGCT^C
520 GAGCTC
TaqI 156 TCGA T^CGA
344 TCGA
TfiI 158 GATTC G^A[AT]TC
164 GAATC
TthHB8I 156 TCGA T^CGA
344 TCGA
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HgaI
ItaI MaeIII MboI MnlI MspA1I
MvaI MwoI NdeII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
TseI Tsp45I TspRI