Restriction map for
pF1KE0936
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT14I EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KspI MaeIII MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tsp45I Tth111I
XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 75 GGTACC G^GTACC
AcsI 342 GAATTC [GA]^AATT[CT]
AcyI 1216 GACGCC G[GA]^CG[CT]C
ApaI 487 GGGCCC GGGCC^C
ApoI 342 GAATTC [GA]^AATT[CT]
Asp718I 75 GGTACC G^GTACC
BanI 75 GGTACC G^G[CT][GA]CC
BclI 185 TGATCA T^GATCA
BglI 769 GCCAACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1216 GACGCC G[GA]^CG[CT]C
BsaWI 1148 ACCGGA [AT]^CCGG[AT]
BsgI 379 GTGCAG GTGCAG(16/14)
BsmAI 535 GTCTC GTCTC(1/5)
BsmBI 534 CGTCTC CGTCTC(1/5)
BspHI 338 TCATGA T^CATGA
BspMI 177 GCAGGT ACCTGC(4/8)
BsrBI 985 GAGCGG CCGCTC(-3/-3)
BsrDI 316 GCAATG GCAATG(2/0)
CfoI 527 GCGC GCG^C
DraIII 1192 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 753 GACCTCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 342 GAATTC G^AATTC
FbaI 185 TGATCA T^GATCA
HaeII 526 AGCGCC [GA]GCGC^[CT]
HhaI 527 GCGC GCG^C
Hin1I 1216 GACGCC G[GA]^CG[CT]C
HinP1I 527 GCGC G^CGC
KpnI 75 GGTACC GGTAC^C
MaeII 304 ACGT A^CGT
MseI 274 TTAA T^TAA
PflMI 583 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 518 CTGCAG CTGCA^G
RcaI 338 TCATGA T^CATGA
SfcI 518 CTGCAG C^T[GA][CT]AG
SmaI 32 CCCGGG CCC^GGG
StuI 801 AGGCCT AGG^CCT
Tru9I 274 TTAA T^TAA
Van91I 583 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 462 TCTAGA T^CTAGA
XcmI 662 CCATGTCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 32 CCCGGG C^CCGGG
XmaI 32 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 49 GTCTAC GT^[AC][GT]AC
265 GTCTAC
AlwNI 962 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1228 CAGGAGCTG
AspHI 657 GAGCTC G[AT]GC[AT]^C
1186 GAGCTC
AvaI 32 CCCGGG C^[CT]CG[GA]G
932 CCCGAG
BbsI 419 GAAGAC GAAGAC(2/6)
721 GAAGAC
BpuAI 419 GAAGAC GAAGAC(2/6)
721 GAAGAC
BsaBI 111 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
405 GATGGACATC
BsiHKAI 657 GAGCTC G[AT]GC[AT]^C
1186 GAGCTC
BsoBI 32 CCCGGG C^[CT]CG[GA]G
932 CCCGAG
BstYI 903 AGATCC [GA]^GATC[CT]
1163 AGATCC
EarI 292 GAAGAG CTCTTC(1/4)
704 GAAGAG
EcoNI 804 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1167 CCTAGAGGAGG
FauI 257 CCCGC CCCGC(4/6)
785 GCGGG
HphI 1179 GGTGA GGTGA(8/7)
1197 GGTGA
Ksp632I 292 GAAGAG CTCTTC(1/4)
704 GAAGAG
MamI 111 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
405 GATGGACATC
MflI 903 AGATCC [GA]^GATC[CT]
1163 AGATCC
MspA1I 677 CCGCTG C[AC]G^C[GT]G
783 CAGCGG
PpuMI 751 GGGACCT [GA]G^G[AT]CC[CT]
928 GGGACCC
SacI 657 GAGCTC GAGCT^C
1186 GAGCTC
Tsp509I 190 AATT ^AATT
343 AATT
XhoII 903 AGATCC [GA]^GATC[CT]
1163 AGATCC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII ItaI MaeI MboI
MboII MluNI MnlI MscI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI TseI
TspRI TthHB8I