Restriction map for
pF1KE0932
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbrPI BbsI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BstAPI BstBI BstEII
BstPI BstUI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HgaI HincII HindII HpaI KpnI
KspI MaeII MamI MluI MluNI
MroI MscI MslI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StyI SwaI Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 1003 GTATAC GT^[AC][GT]AC
AcyI 411 GGCGCC G[GA]^CG[CT]C
Alw44I 673 GTGCAC G^TGCAC
AlwNI 560 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 792 GGGCCC GGGCC^C
ApaLI 673 GTGCAC G^TGCAC
AvaI 628 CTCGAG C^[CT]CG[GA]G
AvaII 209 GGACC G^G[AT]CC
BamHI 454 GGATCC G^GATCC
BanI 411 GGCGCC G^G[CT][GA]CC
BbeI 411 GGCGCC GGCGC^C
BbvCI 332 CCTCAGC CCTCAGC(-5/-2)
BciVI 1137 GGATAC GTATCC(6/5)
BclI 1040 TGATCA T^GATCA
BfaI 227 CTAG C^TAG
BpmI 57 CTGGAG CTGGAG(16/14)
BsaHI 411 GGCGCC G[GA]^CG[CT]C
BsaI 641 GAGACC GGTCTC(1/5)
BseRI 117 GAGGAG GAGGAG(10/8)
BsgI 481 GTGCAG GTGCAG(16/14)
BsmFI 562 GTCCC GGGAC(10/14)
BsoBI 628 CTCGAG C^[CT]CG[GA]G
BsrBI 1017 CCGCTC CCGCTC(-3/-3)
BsrDI 710 CATTGC GCAATG(2/0)
BsrI 881 CCAGT ACTGG(1/-1)
BssSI 260 CTCGTG CACGAG(-5/-1)
Bst1107I 1003 GTATAC GTA^TAC
BstYI 454 GGATCC [GA]^GATC[CT]
BstZ17I 1003 GTATAC GTA^TAC
CfoI 412 GCGC GCG^C
DraI 834 TTTAAA TTT^AAA
DraIII 107 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 667 CTCTTC CTCTTC(1/4)
FbaI 1040 TGATCA T^GATCA
HaeII 411 GGCGCC [GA]GCGC^[CT]
HhaI 412 GCGC GCG^C
Hin1I 411 GGCGCC G[GA]^CG[CT]C
HinP1I 412 GCGC G^CGC
HphI 914 TCACC GGTGA(8/7)
KasI 411 GGCGCC G^GCGCC
Ksp632I 667 CTCTTC CTCTTC(1/4)
MaeI 227 CTAG C^TAG
MfeI 32 CAATTG C^AATTG
MflI 454 GGATCC [GA]^GATC[CT]
MspA1I 506 CAGCGG C[AC]G^C[GT]G
MunI 32 CAATTG C^AATTG
NarI 411 GGCGCC GG^CGCC
NspI 684 GCATGC [GA]CATG^[CT]
PaeR7I 628 CTCGAG C^TCGAG
PflMI 106 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 727 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 411 GGCGCC GGC^GCC
SmlI 628 CTCGAG C^T[CT][GA]AG
SphI 684 GCATGC GCATG^C
SspI 844 AATATT AAT^ATT
StuI 488 AGGCCT AGG^CCT
Van91I 106 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 596 CCAAGCTTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 628 CTCGAG C^TCGAG
XhoII 454 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 654 GTAC GT^AC
888 GTAC
AspHI 673 GTGCAC G[AT]GC[AT]^C
1199 GAGCAC
BanII 60 GAGCCC G[GA]GC[CT]^C
792 GGGCCC
BcnI 415 CCGGG CC^[GC]GG
427 CCGGG
BsiHKAI 673 GTGCAC G[AT]GC[AT]^C
1199 GAGCAC
BsmBI 345 CGTCTC CGTCTC(1/5)
967 GAGACG
HapII 415 CCGG C^CGG
427 CCGG
HindIII 78 AAGCTT A^AGCTT
598 AAGCTT
HpaII 415 CCGG C^CGG
427 CCGG
MboII 668 TCTTC GAAGA(8/7)
809 TCTTC
MseI 772 TTAA T^TAA
835 TTAA
MspI 415 CCGG C^CGG
427 CCGG
NciI 415 CCGGG CC^[GC]GG
427 CCGGG
PleI 16 GACTC GAGTC(4/5)
649 GACTC
PstI 20 CTGCAG CTGCA^G
310 CTGCAG
RsaI 654 GTAC GT^AC
888 GTAC
SfcI 20 CTGCAG C^T[GA][CT]AG
310 CTGCAG
TaqI 71 TCGA T^CGA
629 TCGA
Tru9I 772 TTAA T^TAA
835 TTAA
TthHB8I 71 TCGA T^CGA
629 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp45I Tsp509I TspRI