Restriction map for
pF1KE0927
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraIII Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeIII MamI MfeI
MluI MroI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI TfiI
Tsp45I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 216 CGCG CG^CG
AcyI 9 GACGCC G[GA]^CG[CT]C
AflIII 588 ACATGT A^C[GA][CT]GT
BamHI 58 GGATCC G^GATCC
BanI 808 GGTGCC G^G[CT][GA]CC
BanII 654 GGGCTC G[GA]GC[CT]^C
BbsI 730 GAAGAC GAAGAC(2/6)
BglII 779 AGATCT A^GATCT
BmyI 654 GGGCTC G[AGT]GC[ACT]^C
BpmI 548 CTGGAG CTGGAG(16/14)
BpuAI 730 GAAGAC GAAGAC(2/6)
BsaHI 9 GACGCC G[GA]^CG[CT]C
BsaI 556 GAGACC GGTCTC(1/5)
BsiEI 659 CGGCCG CG[GA][CT]^CG
BsmI 623 GCATTC GAATGC(1/-1)
Bsp1286I 654 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 588 ACATGT A^CATGT
BstUI 216 CGCG CG^CG
CfoI 465 GCGC GCG^C
DraI 964 TTTAAA TTT^AAA
DrdI 427 GACTTCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 527 CCGTGG C^C[GA][CT]GG
EagI 659 CGGCCG C^GGCCG
EclXI 659 CGGCCG C^GGCCG
Eco52I 659 CGGCCG C^GGCCG
EcoNI 408 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 488 CCAAGG C^C[AT][AT]GG
HgaI 9 GACGC GACGC(5/10)
HhaI 465 GCGC GCG^C
Hin1I 9 GACGCC G[GA]^CG[CT]C
HinP1I 465 GCGC G^CGC
MaeII 143 ACGT A^CGT
MseI 965 TTAA T^TAA
MslI 147 CATTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 216 CGCG CG^CG
PflMI 720 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 365 GGGACCT [GA]G^G[AT]CC[CT]
SapI 615 GAAGAGC GCTCTTC(1/4)
SbfI 408 CCTGCAGG CCTGCA^GG
SfaNI 1056 GATGC GCATC(5/9)
SmlI 691 CTCAAG C^T[CT][GA]AG
Sse8387I 408 CCTGCAGG CCTGCA^GG
StuI 596 AGGCCT AGG^CCT
StyI 488 CCAAGG C^C[AT][AT]GG
Tru9I 965 TTAA T^TAA
Van91I 720 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 403 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1067 CAGCCTCTG
BalI 185 TGGCCA TGG^CCA
320 TGGCCA
BseRI 96 GAGGAG GAGGAG(10/8)
958 GAGGAG
BsmAI 425 GAGAC GTCTC(1/5)
556 GAGAC
BspMI 314 ACCTGC ACCTGC(4/8)
1034 ACCTGC
BsrI 479 ACTGG ACTGG(1/-1)
805 ACTGG
BstYI 58 GGATCC [GA]^GATC[CT]
779 AGATCT
EarI 615 GAAGAG CTCTTC(1/4)
955 GAAGAG
HphI 167 TCACC GGTGA(8/7)
627 TCACC
Ksp632I 615 GAAGAG CTCTTC(1/4)
955 GAAGAG
MflI 58 GGATCC [GA]^GATC[CT]
779 AGATCT
MluNI 185 TGGCCA TGG^CCA
320 TGGCCA
MscI 185 TGGCCA TGG^CCA
320 TGGCCA
PstI 409 CTGCAG CTGCA^G
1073 CTGCAG
SfcI 409 CTGCAG C^T[GA][CT]AG
1073 CTGCAG
SphI 461 GCATGC GCATG^C
831 GCATGC
TspRI 804 CACTG CA[GC]TG[ACGT][ACGT]^
915 CACTG
XhoII 58 GGATCC [GA]^GATC[CT]
779 AGATCT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BfaI BsaJI BsiYI
BslI BsmFI BsrBI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MaeI MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI TaqI TseI TthHB8I
XcmI