Restriction map for
pF1KE0926
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MluI MluNI MroI MscI MspA1I
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 564 GTATAC GT^[AC][GT]AC
AflIII 471 ACATGT A^C[GA][CT]GT
AhdI 1039 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1005 GTGCAC G^TGCAC
ApaI 588 GGGCCC GGGCC^C
ApaLI 1005 GTGCAC G^TGCAC
AspEI 1039 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 36 GGTCC G^G[AT]CC
BamHI 718 GGATCC G^GATCC
BbvCI 722 CCTCAGC CCTCAGC(-5/-2)
BcgI 1053 GCATCGCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 464 AGATCT A^GATCT
BpmI 292 CTCCAG CTGGAG(16/14)
BsgI 544 GTGCAG GTGCAG(16/14)
Bsp1407I 851 TGTACA T^GTACA
BspLU11I 471 ACATGT A^CATGT
BsrDI 766 GCAATG GCAATG(2/0)
BsrGI 851 TGTACA T^GTACA
Bst1107I 564 GTATAC GTA^TAC
BstZ17I 564 GTATAC GTA^TAC
Eam1105I 1039 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MfeI 707 CAATTG C^AATTG
MunI 707 CAATTG C^AATTG
NheI 301 GCTAGC G^CTAGC
PflMI 610 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 958 GAGCTC GAGCT^C
SphI 305 GCATGC GCATG^C
SspBI 851 TGTACA T^GTACA
TaqI 5 TCGA T^CGA
TthHB8I 5 TCGA T^CGA
Van91I 610 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 665 CCATGTCTCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 680 AAATTC [GA]^AATT[CT]
940 AAATTT
AfaI 704 GTAC GT^AC
852 GTAC
ApoI 680 AAATTC [GA]^AATT[CT]
940 AAATTT
BfaI 227 CTAG C^TAG
302 CTAG
BsmAI 549 GTCTC GTCTC(1/5)
669 GTCTC
BspMI 66 GCAGGT ACCTGC(4/8)
78 GCAGGT
BstXI 161 CCAACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
530 CCATCCCAGTGG
DraII 587 GGGGCCC [GA]G^G[ACGT]CC[CT]
588 GGGCCCT
DsaI 167 CCATGG C^C[GA][CT]GG
557 CCATGG
EarI 787 GAAGAG CTCTTC(1/4)
804 GAAGAG
EcoO109I 587 GGGGCCC [GA]G^G[ACGT]CC[CT]
588 GGGCCCT
Ksp632I 787 GAAGAG CTCTTC(1/4)
804 GAAGAG
MaeI 227 CTAG C^TAG
302 CTAG
MaeII 324 ACGT A^CGT
370 ACGT
MseI 44 TTAA T^TAA
188 TTAA
NcoI 167 CCATGG C^CATGG
557 CCATGG
NspI 305 GCATGC [GA]CATG^[CT]
471 ACATGT
PleI 735 GAGTC GAGTC(4/5)
790 GAGTC
RsaI 704 GTAC GT^AC
852 GTAC
TfiI 816 GATTC G^A[AT]TC
887 GAATC
Tru9I 44 TTAA T^TAA
188 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BanII BbsI BbvI BmyI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI XhoII