Restriction map for
pF1KE0925
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BpmI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraII
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AlwI 587 GGATC GGATC(4/5)
AlwNI 194 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 250 ATTAAT AT^TAAT
AsnI 250 ATTAAT AT^TAAT
AspHI 58 GAGCTC G[AT]GC[AT]^C
AvaI 43 CCCGGG C^[CT]CG[GA]G
AvaII 550 GGACC G^G[AT]CC
BalI 662 TGGCCA TGG^CCA
BanI 23 GGTGCC G^G[CT][GA]CC
BfaI 931 CTAG C^TAG
BlpI 238 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 238 GCTAAGC GC^T[ACGT]AGC
BsaBI 742 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 83 GAGGAG GAGGAG(10/8)
BsgI 593 GTGCAG GTGCAG(16/14)
BsiHKAI 58 GAGCTC G[AT]GC[AT]^C
BsoBI 43 CCCGGG C^[CT]CG[GA]G
BspMI 243 GCAGGT ACCTGC(4/8)
BsrDI 8 GCAATG GCAATG(2/0)
BsrI 142 CCAGT ACTGG(1/-1)
BstF5I 309 GGATG GGATG(2/0)
CelII 238 GCTAAGC GC^T[ACGT]AGC
Cfr13I 550 GGACC G^G[ACGT]CC
DraI 340 TTTAAA TTT^AAA
DrdI 40 GACCCCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 737 CCATGG C^C[GA][CT]GG
EaeI 662 TGGCCA [CT]^GGCC[GA]
EcoT14I 737 CCATGG C^C[AT][AT]GG
FokI 309 GGATG GGATG(9/13)
MaeI 931 CTAG C^TAG
MamI 742 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 662 TGGCCA TGG^CCA
MscI 662 TGGCCA TGG^CCA
MslI 304 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 737 CCATGG C^CATGG
NspI 568 GCATGC [GA]CATG^[CT]
PflMI 142 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 211 GACTC GAGTC(4/5)
PshBI 250 ATTAAT AT^TAAT
SacI 58 GAGCTC GAGCT^C
Sau96I 550 GGACC G^G[ACGT]CC
SmaI 43 CCCGGG CCC^GGG
SmlI 269 CTCAAG C^T[CT][GA]AG
SpeI 930 ACTAGT A^CTAGT
SphI 568 GCATGC GCATG^C
StuI 601 AGGCCT AGG^CCT
StyI 737 CCATGG C^C[AT][AT]GG
Van91I 142 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 43 CCCGGG C^CCGGG
XmaI 43 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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BanII 58 GAGCTC G[GA]GC[CT]^C
86 GAGCCC
BbsI 459 GAAGAC GAAGAC(2/6)
49 GTCTTC
BbvI 174 GCAGC GCAGC(8/12)
367 GCAGC
BciVI 620 GGATAC GTATCC(6/5)
741 GGATAC
BmyI 58 GAGCTC G[AGT]GC[ACT]^C
86 GAGCCC
BpuAI 459 GAAGAC GAAGAC(2/6)
49 GTCTTC
BsmAI 325 GAGAC GTCTC(1/5)
519 GAGAC
Bsp1286I 58 GAGCTC G[AGT]GC[ACT]^C
86 GAGCCC
BstNI 178 CCAGG CC^[AT]GG
306 CCAGG
Cac8I 193 GCAGGC GC[ACGT]^[ACGT]GC
568 GCATGC
DpnII 588 GATC ^GATC
748 GATC
EcoRII 178 CCAGG ^CC[AT]GG
306 CCAGG
Fnu4HI 174 GCAGC GC^[ACGT]GC
367 GCAGC
HaeIII 602 GGCC GG^CC
663 GGCC
HapII 14 CCGG C^CGG
44 CCGG
HpaII 14 CCGG C^CGG
44 CCGG
HphI 640 GGTGA GGTGA(8/7)
53 TCACC
ItaI 174 GCAGC GC^[ACGT]GC
367 GCAGC
MaeIII 288 GTTAC ^GT[ACGT]AC
711 GTTAC
MboI 588 GATC ^GATC
748 GATC
MspI 14 CCGG C^CGG
44 CCGG
MvaI 178 CCAGG CC^[AT]GG
306 CCAGG
MwoI 265 GCTACTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
961 GCAAAGAAAGC
NdeII 588 GATC ^GATC
748 GATC
Sau3AI 588 GATC ^GATC
748 GATC
TaqI 408 TCGA T^CGA
648 TCGA
TfiI 71 GATTC G^A[AT]TC
405 GATTC
TseI 174 GCAGC G^C[AT]GC
367 GCAGC
TthHB8I 408 TCGA T^CGA
648 TCGA
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
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AcsI AfaI AluI ApoI BcnI
BsaJI BsiYI BslI BssKI DdeI
HinfI MboII MnlI MseI NciI
NlaIII NlaIV RsaI ScrFI SfaNI
Tru9I Tsp509I TspRI