Restriction map for
pF1KE0920
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SfcI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 111 GTAC GT^AC
AflIII 201 ACACGT A^C[GA][CT]GT
AhdI 556 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 448 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 556 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 583 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 191 TGGCCA TGG^CCA
BanI 75 GGCGCC G^G[CT][GA]CC
BbeI 75 GGCGCC GGCGC^C
BbrPI 202 CACGTG CAC^GTG
BglII 381 AGATCT A^GATCT
BsaAI 202 CACGTG [CT]AC^GT[GA]
BsaWI 215 ACCGGA [AT]^CCGG[AT]
BseRI 421 GAGGAG GAGGAG(10/8)
BsgI 58 CTGCAC GTGCAG(16/14)
BsmBI 171 CGTCTC CGTCTC(1/5)
BssHII 36 GCGCGC G^CGCGC
BstBI 841 TTCGAA TT^CGAA
DrdI 340 GACAGTTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 191 TGGCCA [CT]^GGCC[GA]
Eam1105I 556 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HinfI 103 GAGTC G^A[ACGT]TC
HphI 285 GGTGA GGTGA(8/7)
KasI 75 GGCGCC G^GCGCC
MaeII 203 ACGT A^CGT
MluNI 191 TGGCCA TGG^CCA
MscI 191 TGGCCA TGG^CCA
MspA1I 135 CAGCGG C[AC]G^C[GT]G
NarI 75 GGCGCC GG^CGCC
NspV 841 TTCGAA TT^CGAA
PaeR7I 251 CTCGAG C^TCGAG
PflFI 583 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 103 GAGTC GAGTC(4/5)
PmaCI 202 CACGTG CAC^GTG
PmlI 202 CACGTG CAC^GTG
PpuMI 607 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 111 GTAC GT^AC
SexAI 113 ACCAGGT A^CC[AT]GGT
SfoI 75 GGCGCC GGC^GCC
SfuI 841 TTCGAA TT^CGAA
TspRI 469 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 583 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 251 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 154 GTCTAC GT^[AC][GT]AC
589 GTCTAC
AcyI 75 GGCGCC G[GA]^CG[CT]C
304 GGCGTC
ApaI 120 GGGCCC GGGCC^C
928 GGGCCC
BamHI 635 GGATCC G^GATCC
686 GGATCC
BcnI 79 CCCGG CC^[GC]GG
925 CCGGG
BsaHI 75 GGCGCC G[GA]^CG[CT]C
304 GGCGTC
BsaI 819 GAGACC GGTCTC(1/5)
774 GGTCTC
BsmFI 607 GGGAC GGGAC(10/14)
678 GTCCC
BsrFI 186 GCCGGT [GA]^CCGG[CT]
372 GCCGGT
BstXI 65 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
260 CCATGGAAGTGG
Cfr10I 186 GCCGGT [GA]^CCGG[CT]
372 GCCGGT
DraII 607 GGGACCC [GA]G^G[ACGT]CC[CT]
927 GGGGCCC
EcoNI 414 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
882 CCTGCCCCAGG
EcoO109I 607 GGGACCC [GA]G^G[ACGT]CC[CT]
927 GGGGCCC
HaeII 75 GGCGCC [GA]GCGC^[CT]
570 GGCGCT
HgaI 170 GCGTC GACGC(5/10)
305 GCGTC
Hin1I 75 GGCGCC G[GA]^CG[CT]C
304 GGCGTC
MslI 249 CACTCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
261 CATGGAAGTG
NciI 79 CCCGG CC^[GC]GG
925 CCGGG
SacI 288 GAGCTC GAGCT^C
541 GAGCTC
XcmI 194 CCATCAAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
645 CCATCGCTACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BanII BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI Ksp632I MboI
MboII MflI MnlI MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TseI TthHB8I
XhoII