Restriction map for
pF1KE0919
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BfaI BfrI BglI BglII
BlnI BpuAI BsaBI BsaHI BseAI
BseRI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcsI 430 AAATTC [GA]^AATT[CT]
AflIII 332 ACACGT A^C[GA][CT]GT
AgeI 698 ACCGGT A^CCGGT
Alw44I 355 GTGCAC G^TGCAC
AlwI 459 GGATC GGATC(4/5)
ApaLI 355 GTGCAC G^TGCAC
ApoI 430 AAATTC [GA]^AATT[CT]
Asp700I 144 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 243 GGCACC G^G[CT][GA]CC
BcgI 53 GCATGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 883 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 883 GCTCAGC GC^T[ACGT]AGC
BsaAI 333 CACGTA [CT]AC^GT[GA]
BsaWI 698 ACCGGT [AT]^CCGG[AT]
BsgI 17 CTGCAC GTGCAG(16/14)
BsiEI 75 CGGCCG CG[GA][CT]^CG
BsrBI 706 CCGCTC CCGCTC(-3/-3)
BsrDI 843 CATTGC GCAATG(2/0)
BsrFI 698 ACCGGT [GA]^CCGG[CT]
BstBI 466 TTCGAA TT^CGAA
BstEII 807 GGTCACC G^GT[ACGT]ACC
BstPI 807 GGTCACC G^GT[ACGT]ACC
BstYI 459 GGATCT [GA]^GATC[CT]
CelII 883 GCTCAGC GC^T[ACGT]AGC
CfoI 235 GCGC GCG^C
Cfr10I 698 ACCGGT [GA]^CCGG[CT]
EagI 75 CGGCCG C^GGCCG
EarI 463 CTCTTC CTCTTC(1/4)
EclXI 75 CGGCCG C^GGCCG
Eco52I 75 CGGCCG C^GGCCG
EcoNI 187 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 807 GGTCACC G^GT[ACGT]ACC
EcoT14I 250 CCATGG C^C[AT][AT]GG
HhaI 235 GCGC GCG^C
HinP1I 235 GCGC G^CGC
Ksp632I 463 CTCTTC CTCTTC(1/4)
MaeIII 808 GTCAC ^GT[ACGT]AC
MboII 464 TCTTC GAAGA(8/7)
MflI 459 GGATCT [GA]^GATC[CT]
MslI 245 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 181 CCGCTG C[AC]G^C[GT]G
NcoI 250 CCATGG C^CATGG
NspV 466 TTCGAA TT^CGAA
PflMI 278 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 698 ACCGGT A^CCGGT
SfaNI 692 GCATC GCATC(5/9)
SfuI 466 TTCGAA TT^CGAA
SmaI 65 CCCGGG CCC^GGG
StyI 250 CCATGG C^C[AT][AT]GG
Tsp45I 808 GTCAC ^GT[GC]AC
Tsp509I 431 AATT ^AATT
TspRI 870 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 278 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 794 CCATCCTGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 459 GGATCT [GA]^GATC[CT]
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
XmnI 144 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 69 GGGCCC GGGCC^C
214 GGGCCC
AspHI 355 GTGCAC G[AT]GC[AT]^C
874 GAGCAC
AvaI 65 CCCGGG C^[CT]CG[GA]G
580 CCCGAG
BalI 269 TGGCCA TGG^CCA
363 TGGCCA
BclI 590 TGATCA T^GATCA
722 TGATCA
BsaI 829 GAGACC GGTCTC(1/5)
558 GGTCTC
BsiHKAI 355 GTGCAC G[AT]GC[AT]^C
874 GAGCAC
BsoBI 65 CCCGGG C^[CT]CG[GA]G
580 CCCGAG
FauI 217 CCCGC CCCGC(4/6)
705 CCCGC
FbaI 590 TGATCA T^GATCA
722 TGATCA
HinfI 202 GAGTC G^A[ACGT]TC
452 GACTC
MaeII 130 ACGT A^CGT
334 ACGT
MluNI 269 TGGCCA TGG^CCA
363 TGGCCA
MscI 269 TGGCCA TGG^CCA
363 TGGCCA
PleI 452 GACTC GAGTC(4/5)
202 GAGTC
PpuMI 323 AGGACCC [GA]G^G[AT]CC[CT]
474 GGGACCC
PstI 209 CTGCAG CTGCA^G
799 CTGCAG
SbfI 208 CCTGCAGG CCTGCA^GG
798 CCTGCAGG
SmlI 454 CTCAAG C^T[CT][GA]AG
742 CTCAAG
Sse8387I 208 CCTGCAGG CCTGCA^GG
798 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfcI
TaqI TseI TthHB8I