##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaJI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeIII      HgaI        HincII      
HindII      HpaI        HphI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        MvaI        MvnI        NciI        NcoI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfuI        
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AciI           10  CCGC                 CCGC(-3/-1)
AcyI           15  GGCGCC               G[GA]^CG[CT]C
BanI           15  GGCGCC               G^G[CT][GA]CC
BbeI           15  GGCGCC               GGCGC^C
BbsI           46  GAAGAC               GAAGAC(2/6)
BfaI          383  CTAG                 C^TAG
BglII         186  AGATCT               A^GATCT
BpuAI          46  GAAGAC               GAAGAC(2/6)
BsaHI          15  GGCGCC               G[GA]^CG[CT]C
BsaWI         145  TCCGGT               [AT]^CCGG[AT]
BsmAI         319  GTCTC                GTCTC(1/5)
BsrDI         225  GCAATG               GCAATG(2/0)
BsrFI          12  GCCGGC               [GA]^CCGG[CT]
Cfr10I         12  GCCGGC               [GA]^CCGG[CT]
DraI          124  TTTAAA               TTT^AAA
EcoRI         430  GAATTC               G^AATTC
Hin1I          15  GGCGCC               G[GA]^CG[CT]C
HindIII        94  AAGCTT               A^AGCTT
KasI           15  GGCGCC               G^GCGCC
MaeI          383  CTAG                 C^TAG
MaeIII        230  GTGAC                ^GT[ACGT]AC
MwoI            9  GCCGCCGGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI           12  GCCGGC               GCC^GGC
NarI           15  GGCGCC               GG^CGCC
NdeI          239  CATATG               CA^TATG
NgoMIV         12  GCCGGC               G^CCGGC
NheI          382  GCTAGC               G^CTAGC
NlaIII        473  CATG                 CATG^
NlaIV          15  GGCGCC               GG[ACGT]^[ACGT]CC
SfoI           15  GGCGCC               GGC^GCC
SgrAI          11  CGCCGGCG             C[GA]^CCGG[CT]G
Tsp45I        230  GTGAC                ^GT[GC]AC
TspRI         369  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AcsI          209  AAATTT               [GA]^AATT[CT]
              430  GAATTC              
AlwI          463  GATCC                GGATC(4/5)
              402  GGATC               
ApoI          209  AAATTT               [GA]^AATT[CT]
              430  GAATTC              
CfoI           16  GCGC                 GCG^C
              308  GCGC                
HaeII          15  GGCGCC               [GA]GCGC^[CT]
              307  GGCGCT              
HapII          13  CCGG                 C^CGG
              146  CCGG                
HhaI           16  GCGC                 GCG^C
              308  GCGC                
HinP1I         16  GCGC                 G^CGC
              308  GCGC                
HpaII          13  CCGG                 C^CGG
              146  CCGG                
MspI           13  CCGG                 C^CGG
              146  CCGG                
ScaI          251  AGTACT               AGT^ACT
              299  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AfaI        AluI        BbvI        BstYI       Cac8I       
DdeI        DpnII       Fnu4HI      HinfI       ItaI        
MboI        MboII       MflI        MnlI        MseI        
NdeII       RsaI        Sau3AI      TfiI        Tru9I       
TseI        Tsp509I     XhoII