Restriction map for
pF1KE0648
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BpmI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BsrI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp509I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1021 GTCTAC GT^[AC][GT]AC
AhdI 934 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 934 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1015 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 570 TGGCCA TGG^CCA
BbsI 246 GAAGAC GAAGAC(2/6)
BcgI 510 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 223 CTAG C^TAG
BlpI 561 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 561 GCTGAGC GC^T[ACGT]AGC
BpuAI 246 GAAGAC GAAGAC(2/6)
BsaI 658 GAGACC GGTCTC(1/5)
BsiEI 318 CGACCG CG[GA][CT]^CG
BsmAI 658 GAGAC GTCTC(1/5)
BsrDI 566 GCAATG GCAATG(2/0)
BssHII 254 GCGCGC G^CGCGC
BstXI 158 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 561 GCTGAGC GC^T[ACGT]AGC
DpnII 468 GATC ^GATC
Eam1105I 934 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 531 AAGCTT A^AGCTT
MaeI 223 CTAG C^TAG
MboI 468 GATC ^GATC
MluNI 570 TGGCCA TGG^CCA
MscI 570 TGGCCA TGG^CCA
MseI 971 TTAA T^TAA
NdeII 468 GATC ^GATC
NspI 591 GCATGT [GA]CATG^[CT]
PflFI 1015 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1135 CTGCAG CTGCA^G
SacI 637 GAGCTC GAGCT^C
Sau3AI 468 GATC ^GATC
SfcI 1135 CTGCAG C^T[GA][CT]AG
SfiI 724 GGCCAGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 719 CCCGGG CCC^GGG
StuI 225 AGGCCT AGG^CCT
TaqI 647 TCGA T^CGA
Tru9I 971 TTAA T^TAA
TspRI 765 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1015 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 647 TCGA T^CGA
XmaCI 719 CCCGGG C^CCGGG
XmaI 719 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwNI 55 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
601 CAGACGCTG
ApaI 817 GGGCCC GGGCC^C
1130 GGGCCC
AspHI 637 GAGCTC G[AT]GC[AT]^C
1102 GAGCAC
AvaI 694 CCCGAG C^[CT]CG[GA]G
719 CCCGGG
AviII 545 TGCGCA TGC^GCA
789 TGCGCA
BglI 361 GCCTATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
725 GCCAGGTGGGC
BsaAI 163 TACGTG [CT]AC^GT[GA]
751 TACGTG
BsiHKAI 637 GAGCTC G[AT]GC[AT]^C
1102 GAGCAC
BsoBI 694 CCCGAG C^[CT]CG[GA]G
719 CCCGGG
BssSI 199 CTCGTG CACGAG(-5/-1)
1009 CTCGTG
DsaI 662 CCATGG C^C[GA][CT]GG
1052 CCATGG
EcoT14I 662 CCATGG C^C[AT][AT]GG
1052 CCATGG
FspI 545 TGCGCA TGC^GCA
789 TGCGCA
MslI 414 CACCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
663 CATGGAGGTG
NcoI 662 CCATGG C^CATGG
1052 CCATGG
SfaNI 262 GATGC GCATC(5/9)
323 GCATC
StyI 662 CCATGG C^C[AT][AT]GG
1052 CCATGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrBI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIII NlaIV PflMI PleI RsaI
Sau96I ScrFI SfoI TseI Tsp45I
Van91I XcmI