##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        Tru9I       
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AciI          298  GCGG                 CCGC(-3/-1)
AcsI          365  AAATTC               [GA]^AATT[CT]
AfaI          450  GTAC                 GT^AC
AlwNI         233  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          365  AAATTC               [GA]^AATT[CT]
Asp700I        73  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI         195  GGATAC               GTATCC(6/5)
BfaI          200  CTAG                 C^TAG
BsaJI         356  CCCAGG               C^C[ACGT][ACGT]GG
BsiYI         351  CCATGCCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          351  CCATGCCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         282  GTCTC                GTCTC(1/5)
BspHI         516  TCATGA               T^CATGA
Cac8I         342  GCAAGC               GC[ACGT]^[ACGT]GC
HaeIII         47  GGCC                 GG^CC
HphI           17  TCACC                GGTGA(8/7)
MaeI          200  CTAG                 C^TAG
MaeII         539  ACGT                 A^CGT
MboII         243  GAAGA                GAAGA(8/7)
MspA1I        337  CAGCTG               C[AC]G^C[GT]G
PleI          208  GAGTC                GAGTC(4/5)
PvuII         337  CAGCTG               CAG^CTG
RcaI          516  TCATGA               T^CATGA
RsaI          450  GTAC                 GT^AC
SfaNI         257  GCATC                GCATC(5/9)
SfcI          547  CTACAG               C^T[GA][CT]AG
StuI           46  AGGCCT               AGG^CCT
TaqI          268  TCGA                 T^CGA
TthHB8I       268  TCGA                 T^CGA
XmnI           73  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AccI          210  GTCTAC               GT^[AC][GT]AC
              311  GTAGAC              
BssKI          44  CCAGG                ^CC[ACGT]GG
              357  CCAGG               
BstNI          44  CCAGG                CC^[AT]GG
              357  CCAGG               
EcoRII         44  CCAGG                ^CC[AT]GG
              357  CCAGG               
MvaI           44  CCAGG                CC^[AT]GG
              357  CCAGG               
MwoI          346  GCTGCCCATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              402  GCCTTTCCAGC         
ScrFI          44  CCAGG                CC^[ACGT]GG
              357  CCAGG               
Tsp509I       366  AATT                 ^AATT
              523  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BbvI        DdeI        Fnu4HI      HinfI       
ItaI        MnlI        NlaIII      TfiI        TseI        
TspRI