Restriction map for
pF1KB6516
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KspI MfeI MluI
MunI MvnI MwoI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 655 GGTACC G^GTACC
AccIII 311 TCCGGA T^CCGGA
AciI 250 GCGG CCGC(-3/-1)
AcyI 472 GACGCC G[GA]^CG[CT]C
AlwI 842 GGATC GGATC(4/5)
ApaI 601 GGGCCC GGGCC^C
Asp700I 84 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 655 GGTACC G^GTACC
AspHI 63 GAGCAC G[AT]GC[AT]^C
BanI 655 GGTACC G^G[CT][GA]CC
BbsI 270 GAAGAC GAAGAC(2/6)
BglII 428 AGATCT A^GATCT
BpuAI 270 GAAGAC GAAGAC(2/6)
BsaHI 472 GACGCC G[GA]^CG[CT]C
BsaWI 311 TCCGGA [AT]^CCGG[AT]
BseAI 311 TCCGGA T^CCGGA
BsiHKAI 63 GAGCAC G[AT]GC[AT]^C
BsmAI 212 GAGAC GTCTC(1/5)
BsmFI 74 GGGAC GGGAC(10/14)
BsmI 1069 GCATTC GAATGC(1/-1)
BspEI 311 TCCGGA T^CCGGA
BspHI 845 TCATGA T^CATGA
BspMI 574 ACCTGC ACCTGC(4/8)
BstF5I 859 CATCC GGATG(2/0)
BstYI 428 AGATCT [GA]^GATC[CT]
DraIII 701 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1039 CTCTTC CTCTTC(1/4)
FokI 859 CATCC GGATG(9/13)
HapII 312 CCGG C^CGG
HgaI 472 GACGC GACGC(5/10)
Hin1I 472 GACGCC G[GA]^CG[CT]C
HpaII 312 CCGG C^CGG
KpnI 655 GGTACC GGTAC^C
Ksp632I 1039 CTCTTC CTCTTC(1/4)
MaeII 334 ACGT A^CGT
MflI 428 AGATCT [GA]^GATC[CT]
MroI 311 TCCGGA T^CCGGA
MspA1I 714 CAGCTG C[AC]G^C[GT]G
MspI 312 CCGG C^CGG
NspI 889 GCATGT [GA]CATG^[CT]
PleI 273 GACTC GAGTC(4/5)
PstI 6 CTGCAG CTGCA^G
PvuII 714 CAGCTG CAG^CTG
RcaI 845 TCATGA T^CATGA
SfaNI 451 GATGC GCATC(5/9)
XcmI 766 CCATTTGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 428 AGATCT [GA]^GATC[CT]
XmnI 84 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 25 GGACC G^G[AT]CC
763 GGACC
BalI 537 TGGCCA TGG^CCA
866 TGGCCA
BanII 391 GAGCCC G[GA]GC[CT]^C
601 GGGCCC
BpmI 785 CTCCAG CTGGAG(16/14)
265 CTGGAG
BsaBI 303 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
429 GATCTATATC
DdeI 906 CTCAG C^T[ACGT]AG
917 CTGAG
DpnII 429 GATC ^GATC
843 GATC
DraII 601 GGGCCCC [GA]G^G[ACGT]CC[CT]
641 AGGCCCC
EaeI 537 TGGCCA [CT]^GGCC[GA]
866 TGGCCA
EcoO109I 601 GGGCCCC [GA]G^G[ACGT]CC[CT]
641 AGGCCCC
MaeIII 503 GTTAC ^GT[ACGT]AC
1063 GTTAC
MamI 303 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
429 GATCTATATC
MboI 429 GATC ^GATC
843 GATC
MluNI 537 TGGCCA TGG^CCA
866 TGGCCA
MscI 537 TGGCCA TGG^CCA
866 TGGCCA
MslI 50 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1044 CATTCCAGTG
NdeII 429 GATC ^GATC
843 GATC
PflMI 179 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
858 CCATCCCCTGG
Sau3AI 429 GATC ^GATC
843 GATC
SfcI 6 CTGCAG C^T[GA][CT]AG
852 CTGTAG
Van91I 179 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
858 CCATCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AluI BbvI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DsaI
EcoRII EcoT14I Fnu4HI HaeIII HinfI
HphI ItaI MaeI MboII MnlI
MseI MvaI NcoI NlaIII NlaIV
RsaI Sau96I ScrFI StyI TfiI
Tru9I TseI Tsp509I TspRI