##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BclI        BcnI        BfaI        BfrI        
BglI        BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
ItaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScrFI       
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        TfiI        TseI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AvaII         567  GGTCC                G^G[AT]CC
BbsI          674  GTCTTC               GAAGAC(2/6)
BciVI         369  GTATCC               GTATCC(6/5)
BpuAI         674  GTCTTC               GAAGAC(2/6)
BsaJI         144  CCTTGG               C^C[ACGT][ACGT]GG
BsgI          214  CTGCAC               GTGCAG(16/14)
BsmAI         149  GAGAC                GTCTC(1/5)
BspMI         212  ACCTGC               ACCTGC(4/8)
EcoT14I       144  CCTTGG               C^C[AT][AT]GG
HinfI         519  GAGTC                G^A[ACGT]TC
MaeIII        248  GTAAC                ^GT[ACGT]AC
MfeI          739  CAATTG               C^AATTG
MunI          739  CAATTG               C^AATTG
MwoI          652  GCCATCATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV          57  GGCCCC               GG[ACGT]^[ACGT]CC
NspI          686  ACATGC               [GA]CATG^[CT]
PleI          519  GAGTC                GAGTC(4/5)
ScaI          353  AGTACT               AGT^ACT
SfcI          543  CTGTAG               C^T[GA][CT]AG
StuI          435  AGGCCT               AGG^CCT
StyI          144  CCTTGG               C^C[AT][AT]GG
TspRI          51  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          272  GTAC                 GT^AC
              354  GTAC                
BglII          33  AGATCT               A^GATCT
              158  AGATCT              
BsiYI         325  CCGTTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              494  CCACCATCAGG         
BslI          325  CCGTTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              494  CCACCATCAGG         
BsrDI         657  CATTGC               GCAATG(2/0)
              175  GCAATG              
BsrI          274  ACTGG                ACTGG(1/-1)
              296  CCAGT               
BstYI          33  AGATCT               [GA]^GATC[CT]
              158  AGATCT              
Cfr13I         57  GGCCC                G^G[ACGT]CC
              567  GGTCC               
EarI          589  CTCTTC               CTCTTC(1/4)
              643  CTCTTC              
Ksp632I       589  CTCTTC               CTCTTC(1/4)
              643  CTCTTC              
MflI           33  AGATCT               [GA]^GATC[CT]
              158  AGATCT              
RsaI          272  GTAC                 GT^AC
              354  GTAC                
Sau96I         57  GGCCC                G^G[ACGT]CC
              567  GGTCC               
XhoII          33  AGATCT               [GA]^GATC[CT]
              158  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        Cac8I       DdeI        DpnII       HaeIII      
MboI        MboII       MnlI        MseI        NdeII       
NlaIII      Sau3AI      Tru9I       Tsp509I