Restriction map for
pF1KE0804
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstF5I BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI FokI FseI
HindIII HinfI HpaI KpnI MaeI
MamI MfeI MflI MluI MroI
MseI MslI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 796 GTAC GT^AC
AgeI 78 ACCGGT A^CCGGT
Aor51HI 305 AGCGCT AGC^GCT
AviII 440 TGCGCA TGC^GCA
BbrPI 478 CACGTG CAC^GTG
BbvCI 786 GCTGAGG CCTCAGC(-5/-2)
BcgI 825 CGAGCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 478 CACGTG [CT]AC^GT[GA]
BsaI 859 GGTCTC GGTCTC(1/5)
BsaWI 78 ACCGGT [AT]^CCGG[AT]
BsgI 151 CTGCAC GTGCAG(16/14)
BsmAI 860 GTCTC GTCTC(1/5)
BsrI 650 ACTGG ACTGG(1/-1)
BssHII 605 GCGCGC G^CGCGC
BstAPI 660 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 552 GGTGACC G^GT[ACGT]ACC
BstPI 552 GGTGACC G^GT[ACGT]ACC
BstXI 704 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 60 GATC ^GATC
DrdI 34 GACAGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 305 AGCGCT AGC^GCT
EcoNI 810 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 552 GGTGACC G^GT[ACGT]ACC
EcoT14I 854 CCATGG C^C[AT][AT]GG
FspI 440 TGCGCA TGC^GCA
HincII 721 GTCAAC GT[CT]^[GA]AC
HindII 721 GTCAAC GT[CT]^[GA]AC
KspI 574 CCGCGG CCGC^GG
MaeII 479 ACGT A^CGT
MboI 60 GATC ^GATC
NcoI 854 CCATGG C^CATGG
NdeII 60 GATC ^GATC
NlaIII 855 CATG CATG^
PinAI 78 ACCGGT A^CCGGT
PmaCI 478 CACGTG CAC^GTG
PmlI 478 CACGTG CAC^GTG
PstI 658 CTGCAG CTGCA^G
RsaI 796 GTAC GT^AC
SacII 574 CCGCGG CCGC^GG
SapI 618 GCTCTTC GCTCTTC(1/4)
Sau3AI 60 GATC ^GATC
SexAI 493 ACCTGGT A^CC[AT]GGT
SfcI 658 CTGCAG C^T[GA][CT]AG
SmaI 769 CCCGGG CCC^GGG
StyI 854 CCATGG C^C[AT][AT]GG
TaqI 32 TCGA T^CGA
TspRI 36 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 32 TCGA T^CGA
XmaCI 769 CCCGGG C^CCGGG
XmaI 769 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 223 GGCGCC G[GA]^CG[CT]C
820 GGCGCC
AluI 730 AGCT AG^CT
827 AGCT
AlwNI 661 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
844 CAGTCCCTG
ApaI 129 GGGCCC GGGCC^C
806 GGGCCC
AspHI 97 GTGCTC G[AT]GC[AT]^C
247 GTGCTC
BalI 482 TGGCCA TGG^CCA
701 TGGCCA
BbeI 223 GGCGCC GGCGC^C
820 GGCGCC
BbsI 43 GTCTTC GAAGAC(2/6)
643 GTCTTC
BcnI 769 CCCGG CC^[GC]GG
770 CCGGG
BglI 188 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
520 GCCTGGACGGC
BpmI 21 CTGGAG CTGGAG(16/14)
651 CTGGAG
BpuAI 43 GTCTTC GAAGAC(2/6)
643 GTCTTC
BsaHI 223 GGCGCC G[GA]^CG[CT]C
820 GGCGCC
BsiEI 365 CGGCCG CG[GA][CT]^CG
404 CGGCCG
BsiHKAI 97 GTGCTC G[AT]GC[AT]^C
247 GTGCTC
BspMI 146 ACCTGC ACCTGC(4/8)
556 ACCTGC
DdeI 209 CTCAG C^T[ACGT]AG
787 CTGAG
EagI 365 CGGCCG C^GGCCG
404 CGGCCG
EarI 322 CTCTTC CTCTTC(1/4)
619 CTCTTC
EclXI 365 CGGCCG C^GGCCG
404 CGGCCG
Eco52I 365 CGGCCG C^GGCCG
404 CGGCCG
Hin1I 223 GGCGCC G[GA]^CG[CT]C
820 GGCGCC
KasI 223 GGCGCC G^GCGCC
820 GGCGCC
Ksp632I 322 CTCTTC CTCTTC(1/4)
619 CTCTTC
MluNI 482 TGGCCA TGG^CCA
701 TGGCCA
MscI 482 TGGCCA TGG^CCA
701 TGGCCA
NaeI 347 GCCGGC GCC^GGC
402 GCCGGC
NarI 223 GGCGCC GG^CGCC
820 GGCGCC
NciI 769 CCCGG CC^[GC]GG
770 CCGGG
NgoMIV 347 GCCGGC G^CCGGC
402 GCCGGC
SfaNI 119 GCATC GCATC(5/9)
593 GCATC
SfoI 223 GGCGCC GGC^GCC
820 GGCGCC
Tsp45I 490 GTCAC ^GT[GC]AC
553 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AvaI AvaII BanI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NlaIV Sau96I ScrFI TseI