Restriction map for
pF1KE0769
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstF5I BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FokI FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MseI
MunI NarI NcoI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 785 TCCGGA T^CCGGA
AspHI 814 GAGCAC G[AT]GC[AT]^C
BalI 585 TGGCCA TGG^CCA
BbsI 781 GTCTTC GAAGAC(2/6)
BbvCI 975 CCTCAGC CCTCAGC(-5/-2)
BclI 303 TGATCA T^GATCA
BfaI 725 CTAG C^TAG
BglI 61 GCCTTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 781 GTCTTC GAAGAC(2/6)
BsaWI 785 TCCGGA [AT]^CCGG[AT]
BseAI 785 TCCGGA T^CCGGA
BsiEI 297 CGGCCG CG[GA][CT]^CG
BsiHKAI 814 GAGCAC G[AT]GC[AT]^C
BsmBI 683 GAGACG CGTCTC(1/5)
BsmFI 890 GTCCC GGGAC(10/14)
BspEI 785 TCCGGA T^CCGGA
BsrFI 822 GCCGGC [GA]^CCGG[CT]
BssSI 591 CTCGTG CACGAG(-5/-1)
BstYI 143 AGATCC [GA]^GATC[CT]
Cfr10I 822 GCCGGC [GA]^CCGG[CT]
EagI 297 CGGCCG C^GGCCG
EclXI 297 CGGCCG C^GGCCG
Eco52I 297 CGGCCG C^GGCCG
FbaI 303 TGATCA T^GATCA
HphI 477 GGTGA GGTGA(8/7)
KspI 498 CCGCGG CCGC^GG
MaeI 725 CTAG C^TAG
MaeIII 868 GTTAC ^GT[ACGT]AC
MflI 143 AGATCC [GA]^GATC[CT]
MluNI 585 TGGCCA TGG^CCA
MroI 785 TCCGGA T^CCGGA
MscI 585 TGGCCA TGG^CCA
MslI 444 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 822 GCCGGC GCC^GGC
NgoMIV 822 GCCGGC G^CCGGC
NheI 724 GCTAGC G^CTAGC
PflMI 324 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 576 GGGTCCT [GA]G^G[AT]CC[CT]
SacII 498 CCGCGG CCGC^GG
SapI 99 GCTCTTC GCTCTTC(1/4)
SfaNI 647 GCATC GCATC(5/9)
SmaI 572 CCCGGG CCC^GGG
StuI 542 AGGCCT AGG^CCT
TfiI 426 GAATC G^A[AT]TC
Van91I 324 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 143 AGATCC [GA]^GATC[CT]
XmaCI 572 CCCGGG C^CCGGG
XmaI 572 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 499 CGCG CG^CG
864 CGCG
AlwI 144 GATCC GGATC(4/5)
480 GATCC
AocI 12 CCTGAGG CC^T[ACGT]AGG
199 CCTGAGG
ApaI 232 GGGCCC GGGCC^C
940 GGGCCC
AvaII 138 GGTCC G^G[AT]CC
577 GGTCC
AviII 452 TGCGCA TGC^GCA
749 TGCGCA
BpmI 651 CTCCAG CTGGAG(16/14)
841 CTGGAG
BsaI 514 GAGACC GGTCTC(1/5)
333 GGTCTC
BseRI 202 GAGGAG GAGGAG(10/8)
925 GAGGAG
BsgI 148 CTGCAC GTGCAG(16/14)
504 CTGCAC
BspMI 152 ACCTGC ACCTGC(4/8)
818 ACCTGC
BsrI 54 CCAGT ACTGG(1/-1)
732 CCAGT
BstAPI 150 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
752 GCACGCAGTGC
BstUI 499 CGCG CG^CG
864 CGCG
Bsu36I 12 CCTGAGG CC^T[ACGT]AGG
199 CCTGAGG
DraIII 33 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
753 CACGCAGTG
DsaI 79 CCACGG C^C[GA][CT]GG
498 CCGCGG
EarI 100 CTCTTC CTCTTC(1/4)
883 CTCTTC
Eco81I 12 CCTGAGG CC^T[ACGT]AGG
199 CCTGAGG
FspI 452 TGCGCA TGC^GCA
749 TGCGCA
Ksp632I 100 CTCTTC CTCTTC(1/4)
883 CTCTTC
MvnI 499 CGCG CG^CG
864 CGCG
PleI 963 GACTC GAGTC(4/5)
391 GAGTC
PstI 523 CTGCAG CTGCA^G
1003 CTGCAG
SexAI 34 ACCTGGT A^CC[AT]GGT
776 ACCAGGT
SfcI 523 CTGCAG C^T[GA][CT]AG
1003 CTGCAG
TaqI 72 TCGA T^CGA
896 TCGA
TthHB8I 72 TCGA T^CGA
896 TCGA
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwNI AvaI BanI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrBI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI TseI TspRI