Restriction map for
pF1KE0749
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AseI AsnI Asp700I Asp718I
AspEI AvrII BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsgI BsiWI BsmBI BsmI BspDI
BspHI BspLU11I BsrDI BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HinfI HpaI KpnI KspI
MaeI MamI MfeI MluI MunI
NcoI NdeI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tsp509I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
ApaI 40 GGGCCC GGGCC^C
AscI 438 GGCGCGCC GG^CGCGCC
AspI 592 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 281 TGCGCA TGC^GCA
BalI 947 TGGCCA TGG^CCA
BamHI 644 GGATCC G^GATCC
BbeI 418 GGCGCC GGCGC^C
BbrPI 208 CACGTG CAC^GTG
BglI 242 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 208 CACGTG [CT]AC^GT[GA]
BsaI 783 GGTCTC GGTCTC(1/5)
BsmAI 784 GTCTC GTCTC(1/5)
Bsp1407I 626 TGTACA T^GTACA
BsrFI 381 GCCGGC [GA]^CCGG[CT]
BsrGI 626 TGTACA T^GTACA
BsrI 332 ACTGG ACTGG(1/-1)
BstEII 225 GGTGACC G^GT[ACGT]ACC
BstPI 225 GGTGACC G^GT[ACGT]ACC
Cfr10I 381 GCCGGC [GA]^CCGG[CT]
DdeI 834 CTCAG C^T[ACGT]AG
DraII 389 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 889 GACGGGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 663 CCACGG C^C[GA][CT]GG
EagI 841 CGGCCG C^GGCCG
EclXI 841 CGGCCG C^GGCCG
Eco52I 841 CGGCCG C^GGCCG
EcoO109I 389 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 225 GGTGACC G^GT[ACGT]ACC
EcoT14I 86 CCAAGG C^C[AT][AT]GG
FspI 281 TGCGCA TGC^GCA
KasI 418 GGCGCC G^GCGCC
MluNI 947 TGGCCA TGG^CCA
MscI 947 TGGCCA TGG^CCA
MseI 512 TTAA T^TAA
MslI 618 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 381 GCCGGC GCC^GGC
NarI 418 GGCGCC GG^CGCC
NgoMIV 381 GCCGGC G^CCGGC
PflFI 592 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 677 CCACCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 208 CACGTG CAC^GTG
PmlI 208 CACGTG CAC^GTG
PpuMI 389 AGGACCT [GA]G^G[AT]CC[CT]
PstI 76 CTGCAG CTGCA^G
SacI 550 GAGCTC GAGCT^C
SapI 768 GCTCTTC GCTCTTC(1/4)
SexAI 119 ACCAGGT A^CC[AT]GGT
SfcI 76 CTGCAG C^T[GA][CT]AG
SfoI 418 GGCGCC GGC^GCC
SspBI 626 TGTACA T^GTACA
StyI 86 CCAAGG C^C[AT][AT]GG
Tru9I 512 TTAA T^TAA
Tth111I 592 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 677 CCACCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 160 GTCTAC GT^[AC][GT]AC
598 GTCTAC
AccIII 544 TCCGGA T^CCGGA
773 TCCGGA
AfaI 117 GTAC GT^AC
627 GTAC
Alw44I 499 GTGCAC G^TGCAC
924 GTGCAC
AlwNI 457 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
799 CAGCAGCTG
ApaLI 499 GTGCAC G^TGCAC
924 GTGCAC
AvaI 376 CCCGAG C^[CT]CG[GA]G
620 CCCGAG
BseAI 544 TCCGGA T^CCGGA
773 TCCGGA
BseRI 745 GAGGAG GAGGAG(10/8)
777 GAGGAG
BsiEI 254 CGACCG CG[GA][CT]^CG
841 CGGCCG
BsoBI 376 CCCGAG C^[CT]CG[GA]G
620 CCCGAG
BspEI 544 TCCGGA T^CCGGA
773 TCCGGA
DraIII 208 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
678 CACCGTGTG
EarI 394 CTCTTC CTCTTC(1/4)
769 CTCTTC
HaeII 418 GGCGCC [GA]GCGC^[CT]
668 GGCGCT
HgaI 314 GCGTC GACGC(5/10)
896 GCGTC
Ksp632I 394 CTCTTC CTCTTC(1/4)
769 CTCTTC
MaeIII 226 GTGAC ^GT[ACGT]AC
911 GTGAC
MroI 544 TCCGGA T^CCGGA
773 TCCGGA
PvuII 486 CAGCTG CAG^CTG
802 CAGCTG
RsaI 117 GTAC GT^AC
627 GTAC
SfaNI 876 GATGC GCATC(5/9)
176 GCATC
SmlI 553 CTCAAG C^T[CT][GA]AG
586 CTCAAG
Tsp45I 226 GTGAC ^GT[GC]AC
911 GTGAC
TspRI 131 CAGTG CA[GC]TG[ACGT][ACGT]^
478 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrBI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DpnII EaeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI TaqI TseI TthHB8I
XhoII