Restriction map for
pF1KE0740
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1286I BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AflIII 721 ACATGT A^C[GA][CT]GT
AlwI 51 GGATC GGATC(4/5)
AseI 181 ATTAAT AT^TAAT
AsnI 181 ATTAAT AT^TAAT
AvaI 22 CCCGAG C^[CT]CG[GA]G
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
BglII 572 AGATCT A^GATCT
BsaAI 700 CACGTA [CT]AC^GT[GA]
BseRI 76 GAGGAG GAGGAG(10/8)
BsoBI 22 CCCGAG C^[CT]CG[GA]G
Bsp1407I 464 TGTACA T^GTACA
BspLU11I 721 ACATGT A^CATGT
BsrDI 243 CATTGC GCAATG(2/0)
BsrGI 464 TGTACA T^GTACA
BstAPI 1112 GCAAAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 1189 TTTAAA TTT^AAA
DraIII 894 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
MaeII 701 ACGT A^CGT
NdeI 729 CATATG CA^TATG
NheI 1176 GCTAGC G^CTAGC
PpuMI 106 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 181 ATTAAT AT^TAAT
PstI 972 CTGCAG CTGCA^G
SfcI 972 CTGCAG C^T[GA][CT]AG
SmlI 71 CTCAAG C^T[CT][GA]AG
SphI 1153 GCATGC GCATG^C
SspBI 464 TGTACA T^GTACA
TaqI 1222 TCGA T^CGA
TthHB8I 1222 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 148 GCGG CCGC(-3/-1)
1180 GCGG
AcyI 3 GGCGTC G[GA]^CG[CT]C
18 GACGCC
AocI 594 CCTGAGG CC^T[ACGT]AGG
856 CCTGAGG
Asp700I 906 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1209 GAAAACTTTC
AvaII 34 GGACC G^G[AT]CC
107 GGTCC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
18 GACGCC
BsrI 619 ACTGG ACTGG(1/-1)
882 CCAGT
BstF5I 144 GGATG GGATG(2/0)
285 GGATG
BstYI 51 GGATCT [GA]^GATC[CT]
572 AGATCT
Bsu36I 594 CCTGAGG CC^T[ACGT]AGG
856 CCTGAGG
Eco81I 594 CCTGAGG CC^T[ACGT]AGG
856 CCTGAGG
FokI 144 GGATG GGATG(9/13)
285 GGATG
HgaI 18 GACGC GACGC(5/10)
4 GCGTC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
18 GACGCC
HphI 559 GGTGA GGTGA(8/7)
622 GGTGA
MflI 51 GGATCT [GA]^GATC[CT]
572 AGATCT
SspI 312 AATATT AAT^ATT
401 AATATT
XhoII 51 GGATCT [GA]^GATC[CT]
572 AGATCT
XmnI 906 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1209 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsgI BsiYI BslI
BsmFI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI HaeIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI