Restriction map for
pF1KE0734
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BanI BbeI BbvCI BciVI
BclI BcnI BfrI BglI BglII
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cac8I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MflI MluI MroI MunI MvnI
MwoI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 518 GTCGAC GT^[AC][GT]AC
AfaI 261 GTAC GT^AC
AvaI 1049 CTCGAG C^[CT]CG[GA]G
AvrII 708 CCTAGG C^CTAGG
BalI 569 TGGCCA TGG^CCA
BbrPI 236 CACGTG CAC^GTG
BcgI 1089 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 708 CCTAGG C^CTAGG
BlpI 1252 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1252 GCTCAGC GC^T[ACGT]AGC
BsaBI 429 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 287 GTGCAG GTGCAG(16/14)
BsmFI 120 GGGAC GGGAC(10/14)
BsoBI 1049 CTCGAG C^[CT]CG[GA]G
BspMI 289 GCAGGT ACCTGC(4/8)
BssKI 749 CCTGG ^CC[ACGT]GG
BstBI 1097 TTCGAA TT^CGAA
BstNI 749 CCTGG CC^[AT]GG
BstXI 344 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1252 GCTCAGC GC^T[ACGT]AGC
CfoI 1257 GCGC GCG^C
DraII 120 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 120 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 749 CCTGG ^CC[AT]GG
EcoRV 785 GATATC GAT^ATC
HaeII 1256 AGCGCC [GA]GCGC^[CT]
HapII 687 CCGG C^CGG
HhaI 1257 GCGC GCG^C
HinP1I 1257 GCGC G^CGC
HincII 518 GTCGAC GT[CT]^[GA]AC
HindII 518 GTCGAC GT[CT]^[GA]AC
HpaII 687 CCGG C^CGG
MamI 429 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 569 TGGCCA TGG^CCA
MscI 569 TGGCCA TGG^CCA
MslI 1159 CACTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 439 CAGCTG C[AC]G^C[GT]G
MspI 687 CCGG C^CGG
MvaI 749 CCTGG CC^[AT]GG
NdeI 715 CATATG CA^TATG
NspI 540 GCATGT [GA]CATG^[CT]
NspV 1097 TTCGAA TT^CGAA
PaeR7I 1049 CTCGAG C^TCGAG
PleI 758 GACTC GAGTC(4/5)
PmaCI 236 CACGTG CAC^GTG
PmlI 236 CACGTG CAC^GTG
PpuMI 120 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 439 CAGCTG CAG^CTG
RsaI 261 GTAC GT^AC
SalI 518 GTCGAC G^TCGAC
ScrFI 749 CCTGG CC^[ACGT]GG
SfuI 1097 TTCGAA TT^CGAA
TfiI 1027 GATTC G^A[AT]TC
Tsp509I 795 AATT ^AATT
XhoI 1049 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 65 GTGCAC G^TGCAC
463 GTGCAC
AlwI 1144 GATCC GGATC(4/5)
1071 GGATC
ApaLI 65 GTGCAC G^TGCAC
463 GTGCAC
BbsI 414 GAAGAC GAAGAC(2/6)
1245 GAAGAC
BpmI 1004 CTCCAG CTGGAG(16/14)
352 CTGGAG
BpuAI 414 GAAGAC GAAGAC(2/6)
1245 GAAGAC
BsaI 990 GAGACC GGTCTC(1/5)
298 GGTCTC
BseRI 90 CTCCTC GAGGAG(10/8)
850 GAGGAG
BsmAI 990 GAGAC GTCTC(1/5)
299 GTCTC
DpnII 1072 GATC ^GATC
1144 GATC
EaeI 569 TGGCCA [CT]^GGCC[GA]
1075 CGGCCA
EarI 928 CTCTTC CTCTTC(1/4)
1094 CTCTTC
HinfI 758 GACTC G^A[ACGT]TC
1027 GATTC
HphI 292 GGTGA GGTGA(8/7)
799 GGTGA
Ksp632I 928 CTCTTC CTCTTC(1/4)
1094 CTCTTC
MaeIII 395 GTGAC ^GT[ACGT]AC
800 GTGAC
MboI 1072 GATC ^GATC
1144 GATC
NcoI 50 CCATGG C^CATGG
344 CCATGG
NdeII 1072 GATC ^GATC
1144 GATC
SacI 169 GAGCTC GAGCT^C
355 GAGCTC
Sau3AI 1072 GATC ^GATC
1144 GATC
SfaNI 666 GCATC GCATC(5/9)
1036 GCATC
SmlI 760 CTCAAG C^T[CT][GA]AG
1049 CTCGAG
Tsp45I 395 GTGAC ^GT[GC]AC
800 GTGAC
TspRI 70 CACTG CA[GC]TG[ACGT][ACGT]^
285 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI AspHI AvaII BanII BbvI
BfaI BmyI BsaAI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrI BstF5I
Cfr13I DdeI DsaI EcoT14I Fnu4HI
FokI HaeIII ItaI MaeI MaeII
MboII MnlI MseI NlaIII NlaIV
Sau96I StyI TaqI Tru9I TseI
TthHB8I