Restriction map for
pF1KE0726
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BalI BanII
BbeI BbvI BcgI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BsiEI
BsiHKAI BsiWI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BstBI BstF5I BstUI BstXI CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI ItaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI TseI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 807 GTATAC GT^[AC][GT]AC
AflII 1219 CTTAAG C^TTAAG
AhdI 600 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 705 CCTTAGG CC^T[ACGT]AGG
AspEI 600 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 700 GGATCC G^GATCC
BanI 659 GGCACC G^G[CT][GA]CC
BbrPI 455 CACGTG CAC^GTG
BbvCI 172 CCTCAGC CCTCAGC(-5/-2)
BciVI 446 GTATCC GTATCC(6/5)
BfaI 116 CTAG C^TAG
BfrI 1219 CTTAAG C^TTAAG
BmyI 710 GGGCAC G[AGT]GC[ACT]^C
BsaJI 5 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 871 TCCGGT [AT]^CCGG[AT]
BseRI 52 GAGGAG GAGGAG(10/8)
BsgI 1066 GTGCAG GTGCAG(16/14)
BsmBI 95 GAGACG CGTCTC(1/5)
BsmFI 3 GTCCC GGGAC(10/14)
BsmI 127 GCATTC GAATGC(1/-1)
Bsp1286I 710 GGGCAC G[AGT]GC[ACT]^C
BspHI 375 TCATGA T^CATGA
BspLU11I 1174 ACATGT A^CATGT
BsrDI 889 CATTGC GCAATG(2/0)
BssKI 6 CCAGG ^CC[ACGT]GG
BssSI 228 CACGAG CACGAG(-5/-1)
Bst1107I 807 GTATAC GTA^TAC
BstEII 15 GGTGACC G^GT[ACGT]ACC
BstNI 6 CCAGG CC^[AT]GG
BstPI 15 GGTGACC G^GT[ACGT]ACC
BstYI 700 GGATCC [GA]^GATC[CT]
BstZ17I 807 GTATAC GTA^TAC
Bsu36I 705 CCTTAGG CC^T[ACGT]AGG
Cac8I 407 GCAGGC GC[ACGT]^[ACGT]GC
DrdI 526 GACGATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 600 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 705 CCTTAGG CC^T[ACGT]AGG
EcoO65I 15 GGTGACC G^GT[ACGT]ACC
EcoRII 6 CCAGG ^CC[AT]GG
HaeIII 651 GGCC GG^CC
HgaI 163 GACGC GACGC(5/10)
HindIII 683 AAGCTT A^AGCTT
MaeI 116 CTAG C^TAG
MflI 700 GGATCC [GA]^GATC[CT]
MspA1I 664 CAGCGG C[AC]G^C[GT]G
MvaI 6 CCAGG CC^[AT]GG
NdeI 964 CATATG CA^TATG
PmaCI 455 CACGTG CAC^GTG
PmlI 455 CACGTG CAC^GTG
RcaI 375 TCATGA T^CATGA
SbfI 404 CCTGCAGG CCTGCA^GG
ScrFI 6 CCAGG CC^[ACGT]GG
Sse8387I 404 CCTGCAGG CCTGCA^GG
TaqI 729 TCGA T^CGA
TthHB8I 729 TCGA T^CGA
XhoII 700 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 812 CCGC CCGC(-3/-1)
666 GCGG
AflIII 454 ACACGT A^C[GA][CT]GT
1174 ACATGT
AlwI 701 GATCC GGATC(4/5)
700 GGATC
AvaII 59 GGACC G^G[AT]CC
69 GGACC
BsaAI 455 CACGTG [CT]AC^GT[GA]
629 TACGTG
BspMI 324 ACCTGC ACCTGC(4/8)
403 ACCTGC
BstAPI 847 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1059 GCATGTTGTGC
DpnII 373 GATC ^GATC
701 GATC
HapII 178 CCGG C^CGG
872 CCGG
HpaII 178 CCGG C^CGG
872 CCGG
MaeIII 16 GTGAC ^GT[ACGT]AC
458 GTGAC
MboI 373 GATC ^GATC
701 GATC
MspI 178 CCGG C^CGG
872 CCGG
NdeII 373 GATC ^GATC
701 GATC
NspI 1059 GCATGT [GA]CATG^[CT]
1174 ACATGT
PstI 326 CTGCAG CTGCA^G
405 CTGCAG
Sau3AI 373 GATC ^GATC
701 GATC
SmlI 462 CTCAAG C^T[CT][GA]AG
1219 CTTAAG
Tsp45I 16 GTGAC ^GT[GC]AC
458 GTGAC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI ApoI BbsI BpmI
BpuAI BsiYI BslI BsmAI BsrI
Cfr13I DdeI HinfI HphI MaeII
MboII MnlI MseI MslI MwoI
NlaIII NlaIV PleI Sau96I SfaNI
SfcI TfiI Tru9I Tsp509I TspRI