Restriction map for
pF1KE0724
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AclI AcsI AflII AhdI
AlwI Aor51HI ApoI AscI AseI
AsnI AspEI AspI AviII BamHI
BbrPI BbsI BciVI BfrI BglII
BpuAI BsiWI Bsp1407I BspDI BspHI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
ClaI DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FspI HincII HindII HindIII HpaI
KspI MaeIII MfeI MflI MluI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 788 GGTACC G^GTACC
AccI 313 GTCTAC GT^[AC][GT]AC
AccIII 89 TCCGGA T^CCGGA
AflIII 719 ACATGT A^C[GA][CT]GT
AgeI 269 ACCGGT A^CCGGT
Alw44I 466 GTGCAC G^TGCAC
AlwNI 1157 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 307 CCTCAGG CC^T[ACGT]AGG
ApaLI 466 GTGCAC G^TGCAC
Asp700I 927 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 788 GGTACC G^GTACC
AvrII 183 CCTAGG C^CTAGG
BbeI 884 GGCGCC GGCGC^C
BbvCI 1045 CCTCAGC CCTCAGC(-5/-2)
BclI 446 TGATCA T^GATCA
BfaI 184 CTAG C^TAG
BglI 632 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 183 CCTAGG C^CTAGG
BlpI 285 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 285 GCTCAGC GC^T[ACGT]AGC
BsaBI 432 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 595 GAGACC GGTCTC(1/5)
BseAI 89 TCCGGA T^CCGGA
BsgI 256 CTGCAC GTGCAG(16/14)
BsmBI 31 GAGACG CGTCTC(1/5)
BsmI 1113 GAATGC GAATGC(1/-1)
BspEI 89 TCCGGA T^CCGGA
BspLU11I 719 ACATGT A^CATGT
BsrBI 784 GAGCGG CCGCTC(-3/-3)
BsrI 983 ACTGG ACTGG(1/-1)
BssHII 646 GCGCGC G^CGCGC
BstXI 347 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 307 CCTCAGG CC^T[ACGT]AGG
CelII 285 GCTCAGC GC^T[ACGT]AGC
CpoI 324 CGGTCCG CG^G[AT]CCG
EagI 1130 CGGCCG C^GGCCG
EclXI 1130 CGGCCG C^GGCCG
Eco52I 1130 CGGCCG C^GGCCG
Eco81I 307 CCTCAGG CC^T[ACGT]AGG
EcoNI 672 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 446 TGATCA T^GATCA
FseI 1131 GGCCGGCC GGCCGG^CC
HinfI 192 GAATC G^A[ACGT]TC
HphI 935 TCACC GGTGA(8/7)
KasI 884 GGCGCC G^GCGCC
KpnI 788 GGTACC GGTAC^C
MaeI 184 CTAG C^TAG
MamI 432 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 89 TCCGGA T^CCGGA
NarI 884 GGCGCC GG^CGCC
NcoI 347 CCATGG C^CATGG
PinAI 269 ACCGGT A^CCGGT
RsrII 324 CGGTCCG CG^G[AT]CCG
SapI 930 GCTCTTC GCTCTTC(1/4)
SfoI 884 GGCGCC GGC^GCC
SphI 543 GCATGC GCATG^C
TfiI 192 GAATC G^A[AT]TC
Tsp509I 224 AATT ^AATT
TspRI 954 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 927 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 301 GGCGTC G[GA]^CG[CT]C
884 GGCGCC
BalI 572 TGGCCA TGG^CCA
860 TGGCCA
BcgI 146 GCAAGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
953 GCAGTGGCTTCG
BsaAI 211 TACGTG [CT]AC^GT[GA]
864 CACGTA
BsaHI 301 GGCGTC G[GA]^CG[CT]C
884 GGCGCC
BsmAI 31 GAGAC GTCTC(1/5)
595 GAGAC
EarI 931 CTCTTC CTCTTC(1/4)
943 CTCTTC
HgaI 140 GCGTC GACGC(5/10)
302 GCGTC
Hin1I 301 GGCGTC G[GA]^CG[CT]C
884 GGCGCC
Ksp632I 931 CTCTTC CTCTTC(1/4)
943 CTCTTC
MluNI 572 TGGCCA TGG^CCA
860 TGGCCA
MscI 572 TGGCCA TGG^CCA
860 TGGCCA
MspA1I 253 CAGCTG C[AC]G^C[GT]G
442 CAGCTG
NaeI 61 GCCGGC GCC^GGC
1132 GCCGGC
NgoMIV 61 GCCGGC G^CCGGC
1132 GCCGGC
NspI 543 GCATGC [GA]CATG^[CT]
719 ACATGT
PpuMI 113 GGGTCCT [GA]G^G[AT]CC[CT]
380 AGGACCT
PstI 1163 CTGCAG CTGCA^G
1231 CTGCAG
PvuII 253 CAGCTG CAG^CTG
442 CAGCTG
SacI 93 GAGCTC GAGCT^C
175 GAGCTC
SfaNI 86 GCATC GCATC(5/9)
659 GCATC
SfcI 1163 CTGCAG C^T[GA][CT]AG
1231 CTGCAG
SmaI 242 CCCGGG CCC^GGG
525 CCCGGG
XmaCI 242 CCCGGG C^CCGGG
525 CCCGGG
XmaI 242 CCCGGG C^CCGGG
525 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SmlI
StyI TaqI TseI TthHB8I