Restriction map for
pF1KE0715
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# NO CUT ENZYMES ( 147 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HaeII HgaI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 574 GACGTC GACGT^C
AccII 39 CGCG CG^CG
AcyI 574 GACGTC G[GA]^CG[CT]C
AfaI 36 GTAC GT^AC
AlwNI 641 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 217 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 305 CCCGGG C^[CT]CG[GA]G
AviII 836 TGCGCA TGC^GCA
BbsI 889 GAAGAC GAAGAC(2/6)
BpuAI 889 GAAGAC GAAGAC(2/6)
BsaHI 574 GACGTC G[GA]^CG[CT]C
BsiEI 851 CGGCCG CG[GA][CT]^CG
BsoBI 305 CCCGGG C^[CT]CG[GA]G
BspHI 830 TCATGA T^CATGA
BsrI 629 CCAGT ACTGG(1/-1)
BstUI 39 CGCG CG^CG
DsaI 761 CCGTGG C^C[GA][CT]GG
EaeI 851 CGGCCG [CT]^GGCC[GA]
EagI 851 CGGCCG C^GGCCG
EclXI 851 CGGCCG C^GGCCG
Eco52I 851 CGGCCG C^GGCCG
FspI 836 TGCGCA TGC^GCA
Hin1I 574 GACGTC G[GA]^CG[CT]C
HindIII 289 AAGCTT A^AGCTT
HinfI 550 GAGTC G^A[ACGT]TC
HphI 604 GGTGA GGTGA(8/7)
MaeII 575 ACGT A^CGT
MslI 645 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 39 CGCG CG^CG
NdeI 212 CATATG CA^TATG
NspI 839 GCATGC [GA]CATG^[CT]
PflFI 217 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 550 GAGTC GAGTC(4/5)
PpuMI 253 AGGACCC [GA]G^G[AT]CC[CT]
PstI 197 CTGCAG CTGCA^G
RcaI 830 TCATGA T^CATGA
RsaI 36 GTAC GT^AC
SacI 16 GAGCTC GAGCT^C
SexAI 617 ACCTGGT A^CC[AT]GGT
SfcI 197 CTGCAG C^T[GA][CT]AG
SmaI 305 CCCGGG CCC^GGG
SphI 839 GCATGC GCATG^C
StuI 173 AGGCCT AGG^CCT
TaqI 383 TCGA T^CGA
Tth111I 217 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 383 TCGA T^CGA
XcmI 693 CCAGAATGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 305 CCCGGG C^CCGGG
XmaI 305 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 16 GAGCTC G[AT]GC[AT]^C
357 GAGCAC
AvaII 30 GGACC G^G[AT]CC
254 GGACC
BanII 16 GAGCTC G[GA]GC[CT]^C
792 GGGCTC
BfaI 46 CTAG C^TAG
370 CTAG
BpmI 298 CTGGAG CTGGAG(16/14)
415 CTGGAG
BsiHKAI 16 GAGCTC G[AT]GC[AT]^C
357 GAGCAC
BsmFI 744 GGGAC GGGAC(10/14)
812 GGGAC
BsrBI 310 GAGCGG CCGCTC(-3/-3)
856 GAGCGG
DpnII 381 GATC ^GATC
870 GATC
DraII 101 AGGCCCT [GA]G^G[ACGT]CC[CT]
253 AGGACCC
EcoO109I 101 AGGCCCT [GA]G^G[ACGT]CC[CT]
253 AGGACCC
MaeI 46 CTAG C^TAG
370 CTAG
MaeIII 7 GTGAC ^GT[ACGT]AC
461 GTGAC
MboI 381 GATC ^GATC
870 GATC
NdeII 381 GATC ^GATC
870 GATC
NlaIII 831 CATG CATG^
840 CATG
Sau3AI 381 GATC ^GATC
870 GATC
Tsp45I 7 GTGAC ^GT[GC]AC
461 GTGAC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI Ksp632I MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIV PvuII Sau96I ScrFI
SfaNI StyI TseI TspRI