Restriction map for
pF1KE0711
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BcgI BciVI BfrI BglII
BlnI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 907 GTCTAC GT^[AC][GT]AC
AccIII 836 TCCGGA T^CCGGA
AfaI 768 GTAC GT^AC
AlwNI 760 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 629 CCCGGG C^[CT]CG[GA]G
BbrPI 595 CACGTG CAC^GTG
BbvCI 711 CCTCAGC CCTCAGC(-5/-2)
BclI 422 TGATCA T^GATCA
BlpI 600 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 600 GCTGAGC GC^T[ACGT]AGC
BsaAI 595 CACGTG [CT]AC^GT[GA]
BsaWI 836 TCCGGA [AT]^CCGG[AT]
BseAI 836 TCCGGA T^CCGGA
BsmFI 642 GGGAC GGGAC(10/14)
BsoBI 629 CCCGGG C^[CT]CG[GA]G
BspEI 836 TCCGGA T^CCGGA
BsrBI 981 CCGCTC CCGCTC(-3/-3)
BsrFI 1124 GCCGGC [GA]^CCGG[CT]
BstXI 893 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 600 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1124 GCCGGC [GA]^CCGG[CT]
DpnII 423 GATC ^GATC
DraIII 592 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 422 TGATCA T^GATCA
HincII 499 GTCAAC GT[CT]^[GA]AC
HindII 499 GTCAAC GT[CT]^[GA]AC
MaeII 596 ACGT A^CGT
MaeIII 8 GTCAC ^GT[ACGT]AC
MboI 423 GATC ^GATC
MroI 836 TCCGGA T^CCGGA
NaeI 1124 GCCGGC GCC^GGC
NdeII 423 GATC ^GATC
NgoMIV 1124 GCCGGC G^CCGGC
PflMI 1166 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 595 CACGTG CAC^GTG
PmlI 595 CACGTG CAC^GTG
PpuMI 229 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 63 CTGCAG CTGCA^G
RsaI 768 GTAC GT^AC
SapI 1005 GCTCTTC GCTCTTC(1/4)
Sau3AI 423 GATC ^GATC
SbfI 62 CCTGCAGG CCTGCA^GG
SfiI 1123 GGCCGGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 629 CCCGGG CCC^GGG
Sse8387I 62 CCTGCAGG CCTGCA^GG
StuI 1187 AGGCCT AGG^CCT
Tsp45I 8 GTCAC ^GT[GC]AC
Van91I 1166 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 629 CCCGGG C^CCGGG
XmaI 629 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
ApaI 241 GGGCCC GGGCC^C
1131 GGGCCC
BalI 18 TGGCCA TGG^CCA
890 TGGCCA
BbsI 74 GAAGAC GAAGAC(2/6)
541 GTCTTC
BfaI 1073 CTAG C^TAG
1193 CTAG
BpuAI 74 GAAGAC GAAGAC(2/6)
541 GTCTTC
BsaI 466 GAGACC GGTCTC(1/5)
1236 GGTCTC
BsmAI 466 GAGAC GTCTC(1/5)
1237 GTCTC
BsrI 792 ACTGG ACTGG(1/-1)
1135 CCAGT
HinfI 77 GACTC G^A[ACGT]TC
644 GACTC
MaeI 1073 CTAG C^TAG
1193 CTAG
MluNI 18 TGGCCA TGG^CCA
890 TGGCCA
MscI 18 TGGCCA TGG^CCA
890 TGGCCA
PleI 77 GACTC GAGTC(4/5)
644 GACTC
PvuII 22 CAGCTG CAG^CTG
623 CAGCTG
SacI 135 GAGCTC GAGCT^C
1149 GAGCTC
SfcI 63 CTGCAG C^T[GA][CT]AG
717 CTACAG
XcmI 107 CCAAGCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
878 CCATGCGTGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NlaIII NlaIV Sau96I
ScrFI SfaNI StyI TseI TspRI