Restriction map for
pF1KE0707
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BamHI BanI BbeI BbsI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MflI MluI MroI MseI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
PshBI Psp1406I RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI StuI SwaI TaqI TfiI
Tru9I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwI 1270 GATCC GGATC(4/5)
AocI 109 CCTGAGG CC^T[ACGT]AGG
AspI 946 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1117 CTCGGG C^[CT]CG[GA]G
AviII 272 TGCGCA TGC^GCA
BalI 731 TGGCCA TGG^CCA
BbvCI 1164 CCTCAGC CCTCAGC(-5/-2)
BcgI 858 GCAGCACGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 627 CCGGG CC^[GC]GG
BglI 593 GCCTGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 5 CTCCTC GAGGAG(10/8)
BsoBI 1117 CTCGGG C^[CT]CG[GA]G
BspHI 737 TCATGA T^CATGA
BsrI 634 CCAGT ACTGG(1/-1)
BstXI 506 CCAGGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 109 CCTGAGG CC^T[ACGT]AGG
DraIII 1188 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 109 CCTGAGG CC^T[ACGT]AGG
FspI 272 TGCGCA TGC^GCA
HaeII 220 GGCGCT [GA]GCGC^[CT]
HphI 516 GGTGA GGTGA(8/7)
MfeI 18 CAATTG C^AATTG
MluNI 731 TGGCCA TGG^CCA
MscI 731 TGGCCA TGG^CCA
MunI 18 CAATTG C^AATTG
NciI 627 CCGGG CC^[GC]GG
NspI 656 GCATGC [GA]CATG^[CT]
PflFI 946 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1224 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 864 CGATCG CGAT^CG
RcaI 737 TCATGA T^CATGA
SacI 349 GAGCTC GAGCT^C
SapI 711 GCTCTTC GCTCTTC(1/4)
SbfI 845 CCTGCAGG CCTGCA^GG
SmlI 145 CTCAAG C^T[CT][GA]AG
SphI 656 GCATGC GCATG^C
Sse8387I 845 CCTGCAGG CCTGCA^GG
Tsp509I 19 AATT ^AATT
Tth111I 946 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1224 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1224 CCACCCCCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AfaI 45 GTAC GT^AC
150 GTAC
Alw44I 490 GTGCAC G^TGCAC
1009 GTGCAC
ApaLI 490 GTGCAC G^TGCAC
1009 GTGCAC
BbrPI 616 CACGTG CAC^GTG
1006 CACGTG
BsaAI 616 CACGTG [CT]AC^GT[GA]
1006 CACGTG
BsiEI 864 CGATCG CG[GA][CT]^CG
945 CGACCG
BsrDI 77 GCAATG GCAATG(2/0)
230 GCAATG
EarI 295 CTCTTC CTCTTC(1/4)
712 CTCTTC
Ksp632I 295 CTCTTC CTCTTC(1/4)
712 CTCTTC
MslI 687 CATGGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
738 CATGAGCGTG
PmaCI 616 CACGTG CAC^GTG
1006 CACGTG
PmlI 616 CACGTG CAC^GTG
1006 CACGTG
PstI 821 CTGCAG CTGCA^G
846 CTGCAG
RsaI 45 GTAC GT^AC
150 GTAC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaII BanII BbvI BmyI BpmI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI