Restriction map for
pF1KE0701
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspI AvaI AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI HphI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 355 GTCTAC GT^[AC][GT]AC
AclI 230 AACGTT AA^CGTT
AhdI 829 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 820 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 193 ATTAAT AT^TAAT
AsnI 193 ATTAAT AT^TAAT
AspEI 829 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 681 GAGCAC G[AT]GC[AT]^C
BalI 164 TGGCCA TGG^CCA
BamHI 125 GGATCC G^GATCC
BanII 455 GAGCCC G[GA]GC[CT]^C
BcnI 312 CCCGG CC^[GC]GG
BglI 498 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 507 GCTGAGC GC^T[ACGT]AGC
BpmI 57 CTCCAG CTGGAG(16/14)
Bpu1102I 507 GCTGAGC GC^T[ACGT]AGC
BsaI 82 GAGACC GGTCTC(1/5)
BseRI 784 GAGGAG GAGGAG(10/8)
BsiHKAI 681 GAGCAC G[AT]GC[AT]^C
BsmAI 82 GAGAC GTCTC(1/5)
Bsp1407I 563 TGTACA T^GTACA
BspMI 222 ACCTGC ACCTGC(4/8)
BsrGI 563 TGTACA T^GTACA
CelII 507 GCTGAGC GC^T[ACGT]AGC
DraI 235 TTTAAA TTT^AAA
DraII 302 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 396 GACAGGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 74 CCATGG C^C[GA][CT]GG
Eam1105I 829 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 302 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 340 GAATTC G^AATTC
EcoT14I 74 CCATGG C^C[AT][AT]GG
EcoT22I 716 ATGCAT ATGCA^T
HapII 313 CCGG C^CGG
HpaII 313 CCGG C^CGG
MluNI 164 TGGCCA TGG^CCA
MscI 164 TGGCCA TGG^CCA
MspI 313 CCGG C^CGG
NciI 312 CCCGG CC^[GC]GG
NcoI 74 CCATGG C^CATGG
NdeI 695 CATATG CA^TATG
NsiI 716 ATGCAT ATGCA^T
NspI 329 GCATGC [GA]CATG^[CT]
PflMI 8 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 302 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 485 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 193 ATTAAT AT^TAAT
Psp1406I 230 AACGTT AA^CGTT
SfaNI 715 GATGC GCATC(5/9)
SphI 329 GCATGC GCATG^C
SspBI 563 TGTACA T^GTACA
StyI 74 CCATGG C^C[AT][AT]GG
Van91I 8 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 564 GTAC GT^AC
597 GTAC
AvaII 303 GGACC G^G[AT]CC
390 GGACC
BbsI 724 GAAGAC GAAGAC(2/6)
837 GTCTTC
BbvI 641 GCAGC GCAGC(8/12)
819 GCAGC
BmyI 455 GAGCCC G[AGT]GC[ACT]^C
681 GAGCAC
BpuAI 724 GAAGAC GAAGAC(2/6)
837 GTCTTC
BsmFI 302 GGGAC GGGAC(10/14)
483 GGGAC
Bsp1286I 455 GAGCCC G[AGT]GC[ACT]^C
681 GAGCAC
BstYI 125 GGATCC [GA]^GATC[CT]
845 AGATCC
Cfr13I 303 GGACC G^G[ACGT]CC
390 GGACC
DpnII 126 GATC ^GATC
846 GATC
EaeI 5 CGGCCA [CT]^GGCC[GA]
164 TGGCCA
HgaI 796 GACGC GACGC(5/10)
95 GCGTC
MaeII 231 ACGT A^CGT
317 ACGT
MboI 126 GATC ^GATC
846 GATC
MflI 125 GGATCC [GA]^GATC[CT]
845 AGATCC
MseI 194 TTAA T^TAA
236 TTAA
NdeII 126 GATC ^GATC
846 GATC
RsaI 564 GTAC GT^AC
597 GTAC
Sau3AI 126 GATC ^GATC
846 GATC
Sau96I 303 GGACC G^G[ACGT]CC
390 GGACC
SfcI 645 CTACAG C^T[GA][CT]AG
651 CTACAG
Tru9I 194 TTAA T^TAA
236 TTAA
TseI 641 GCAGC G^C[AT]GC
819 GCAGC
Tsp45I 748 GTGAC ^GT[GC]AC
812 GTCAC
XcmI 8 CCAGCCTGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
731 CCATGCCACAGCTGG
XhoII 125 GGATCC [GA]^GATC[CT]
845 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I DdeI EcoRII
Fnu4HI FokI HaeIII ItaI MaeIII
MboII MnlI MspA1I MvaI MwoI
NlaIII NlaIV PvuII ScrFI Tsp509I
TspRI