Restriction map for
pF1KE0694
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 762 GTCTAC GT^[AC][GT]AC
AcyI 3 GGCGTC G[GA]^CG[CT]C
ApaI 78 GGGCCC GGGCC^C
BanI 247 GGTGCC G^G[CT][GA]CC
BanII 78 GGGCCC G[GA]GC[CT]^C
BbsI 622 GAAGAC GAAGAC(2/6)
BfaI 947 CTAG C^TAG
BglI 950 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1203 AGATCT A^GATCT
BpuAI 622 GAAGAC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaI 37 GAGACC GGTCTC(1/5)
BsmAI 37 GAGAC GTCTC(1/5)
BstYI 1203 AGATCT [GA]^GATC[CT]
CpoI 60 CGGACCG CG^G[AT]CCG
DsaI 1091 CCACGG C^C[GA][CT]GG
EarI 605 GAAGAG CTCTTC(1/4)
EcoRI 47 GAATTC G^AATTC
EcoT22I 413 ATGCAT ATGCA^T
FauI 41 CCCGC CCCGC(4/6)
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
Ksp632I 605 GAAGAG CTCTTC(1/4)
MaeI 947 CTAG C^TAG
MaeIII 781 GTAAC ^GT[ACGT]AC
MflI 1203 AGATCT [GA]^GATC[CT]
MslI 769 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 946 GCTAGC G^CTAGC
NsiI 413 ATGCAT ATGCA^T
PflMI 26 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 105 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 455 CAGCTG CAG^CTG
RsrII 60 CGGACCG CG^G[AT]CCG
SspI 705 AATATT AAT^ATT
Van91I 26 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1203 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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BalI 700 TGGCCA TGG^CCA
957 TGGCCA
BbvCI 686 CCTCAGC CCTCAGC(-5/-2)
667 GCTGAGG
BmyI 78 GGGCCC G[AGT]GC[ACT]^C
151 GTGCCC
BsmFI 66 GGGAC GGGAC(10/14)
6 GTCCC
Bsp1286I 78 GGGCCC G[AGT]GC[ACT]^C
151 GTGCCC
BspMI 216 ACCTGC ACCTGC(4/8)
978 ACCTGC
DpnII 334 GATC ^GATC
1204 GATC
DraII 78 GGGCCCC [GA]G^G[ACGT]CC[CT]
105 GGGTCCT
EaeI 700 TGGCCA [CT]^GGCC[GA]
957 TGGCCA
EcoO109I 78 GGGCCCC [GA]G^G[ACGT]CC[CT]
105 GGGTCCT
HphI 808 GGTGA GGTGA(8/7)
886 GGTGA
MboI 334 GATC ^GATC
1204 GATC
MluNI 700 TGGCCA TGG^CCA
957 TGGCCA
MscI 700 TGGCCA TGG^CCA
957 TGGCCA
MseI 115 TTAA T^TAA
601 TTAA
MspA1I 455 CAGCTG C[AC]G^C[GT]G
586 CAGCGG
NdeII 334 GATC ^GATC
1204 GATC
NspI 411 GCATGC [GA]CATG^[CT]
655 GCATGC
Sau3AI 334 GATC ^GATC
1204 GATC
SfaNI 442 GATGC GCATC(5/9)
791 GCATC
SfcI 276 CTATAG C^T[GA][CT]AG
583 CTACAG
SphI 411 GCATGC GCATG^C
655 GCATGC
Tru9I 115 TTAA T^TAA
601 TTAA
TspRI 198 CAGTG CA[GC]TG[ACGT][ACGT]^
771 CAGTG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BcnI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI StyI TfiI TseI Tsp509I