Restriction map for
pF1KE0691
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BbvCI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 650 GACGTC GACGT^C
AgeI 611 ACCGGT A^CCGGT
AlwI 377 GGATC GGATC(4/5)
AlwNI 83 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 359 TGCGCA TGC^GCA
BanII 496 GAGCTC G[GA]GC[CT]^C
BbeI 12 GGCGCC GGCGC^C
BbrPI 508 CACGTG CAC^GTG
BbsI 246 GAAGAC GAAGAC(2/6)
BcgI 65 CGACACCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 684 GTATCC GTATCC(6/5)
BpuAI 246 GAAGAC GAAGAC(2/6)
BsaAI 508 CACGTG [CT]AC^GT[GA]
BsaI 537 GGTCTC GGTCTC(1/5)
BsaWI 611 ACCGGT [AT]^CCGG[AT]
BsmBI 529 GAGACG CGTCTC(1/5)
BsrDI 809 CATTGC GCAATG(2/0)
BsrFI 611 ACCGGT [GA]^CCGG[CT]
BstYI 377 GGATCT [GA]^GATC[CT]
Cfr10I 611 ACCGGT [GA]^CCGG[CT]
DraIII 123 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 113 CCACGG C^C[GA][CT]GG
FauI 185 CCCGC CCCGC(4/6)
FspI 359 TGCGCA TGC^GCA
HaeII 12 GGCGCC [GA]GCGC^[CT]
HgaI 531 GACGC GACGC(5/10)
KasI 12 GGCGCC G^GCGCC
MaeIII 427 GTGAC ^GT[ACGT]AC
MflI 377 GGATCT [GA]^GATC[CT]
NarI 12 GGCGCC GG^CGCC
PinAI 611 ACCGGT A^CCGGT
PmaCI 508 CACGTG CAC^GTG
PmlI 508 CACGTG CAC^GTG
SacI 496 GAGCTC GAGCT^C
SfoI 12 GGCGCC GGC^GCC
StuI 152 AGGCCT AGG^CCT
TfiI 172 GATTC G^A[AT]TC
Tsp45I 427 GTGAC ^GT[GC]AC
XcmI 146 CCATTCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 377 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 12 GGCGCC G[GA]^CG[CT]C
650 GACGTC
AfaI 102 GTAC GT^AC
315 GTAC
AspHI 496 GAGCTC G[AT]GC[AT]^C
691 GAGCAC
BanI 12 GGCGCC G^G[CT][GA]CC
420 GGTGCC
BglI 4 GCCGAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
145 GCCATTCAGGC
BsaHI 12 GGCGCC G[GA]^CG[CT]C
650 GACGTC
BseRI 595 GAGGAG GAGGAG(10/8)
724 GAGGAG
BsiHKAI 496 GAGCTC G[AT]GC[AT]^C
691 GAGCAC
BstF5I 570 CATCC GGATG(2/0)
659 GGATG
DpnII 378 GATC ^GATC
840 GATC
FokI 570 CATCC GGATG(9/13)
659 GGATG
Hin1I 12 GGCGCC G[GA]^CG[CT]C
650 GACGTC
HphI 575 TCACC GGTGA(8/7)
698 TCACC
MaeII 509 ACGT A^CGT
651 ACGT
MboI 378 GATC ^GATC
840 GATC
NdeII 378 GATC ^GATC
840 GATC
PpuMI 23 GGGACCC [GA]G^G[AT]CC[CT]
298 GGGACCT
PshAI 52 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
531 GACGCTGGTC
PstI 307 CTGCAG CTGCA^G
325 CTGCAG
RsaI 102 GTAC GT^AC
315 GTAC
Sau3AI 378 GATC ^GATC
840 GATC
SfcI 307 CTGCAG C^T[GA][CT]AG
325 CTGCAG
SmaI 20 CCCGGG CCC^GGG
393 CCCGGG
TaqI 64 TCGA T^CGA
434 TCGA
TspRI 125 CACTG CA[GC]TG[ACGT][ACGT]^
669 CACTG
TthHB8I 64 TCGA T^CGA
434 TCGA
XmaCI 20 CCCGGG C^CCGGG
393 CCCGGG
XmaI 20 CCCGGG C^CCGGG
393 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIV PleI PvuII
Sau96I ScrFI StyI TseI