Restriction map for
pF1KE0689
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AvaII AviII
AvrII BalI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI TspRI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AhdI 592 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 592 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 33 GAGCAC G[AT]GC[AT]^C
BanI 493 GGCACC G^G[CT][GA]CC
BglII 51 AGATCT A^GATCT
BlpI 79 GCTGAGC GC^T[ACGT]AGC
BpmI 88 CTCCAG CTGGAG(16/14)
Bpu1102I 79 GCTGAGC GC^T[ACGT]AGC
BsaI 193 GAGACC GGTCTC(1/5)
BseRI 145 CTCCTC GAGGAG(10/8)
BsgI 562 CTGCAC GTGCAG(16/14)
BsiEI 823 CGGTCG CG[GA][CT]^CG
BsiHKAI 33 GAGCAC G[AT]GC[AT]^C
BsmFI 182 GGGAC GGGAC(10/14)
Bsp1407I 645 TGTACA T^GTACA
BsrGI 645 TGTACA T^GTACA
BsrI 262 ACTGG ACTGG(1/-1)
BstEII 505 GGTTACC G^GT[ACGT]ACC
BstPI 505 GGTTACC G^GT[ACGT]ACC
CelII 79 GCTGAGC GC^T[ACGT]AGC
Cfr13I 224 GGGCC G^G[ACGT]CC
Eam1105I 592 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 140 CTCTTC CTCTTC(1/4)
EcoO65I 505 GGTTACC G^GT[ACGT]ACC
EcoRI 292 GAATTC G^AATTC
EcoT14I 798 CCAAGG C^C[AT][AT]GG
Ksp632I 140 CTCTTC CTCTTC(1/4)
MspA1I 641 CAGCTG C[AC]G^C[GT]G
PflMI 90 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 641 CAGCTG CAG^CTG
SapI 139 GCTCTTC GCTCTTC(1/4)
Sau96I 224 GGGCC G^G[ACGT]CC
SspBI 645 TGTACA T^GTACA
StuI 525 AGGCCT AGG^CCT
StyI 798 CCAAGG C^C[AT][AT]GG
SwaI 273 ATTTAAAT ATTT^AAAT
TaqI 653 TCGA T^CGA
TthHB8I 653 TCGA T^CGA
Van91I 90 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 18 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 277 AAATTT [GA]^AATT[CT]
292 GAATTC
AfaI 207 GTAC GT^AC
646 GTAC
ApoI 277 AAATTT [GA]^AATT[CT]
292 GAATTC
BamHI 42 GGATCC G^GATCC
617 GGATCC
BcnI 200 CCGGG CC^[GC]GG
426 CCGGG
BfaI 19 CTAG C^TAG
622 CTAG
BsaJI 38 CCCAGG C^C[ACGT][ACGT]GG
798 CCAAGG
BsmAI 193 GAGAC GTCTC(1/5)
352 GAGAC
BsrDI 188 GCAATG GCAATG(2/0)
439 GCAATG
BstF5I 379 CATCC GGATG(2/0)
157 GGATG
DraI 274 TTTAAA TTT^AAA
835 TTTAAA
FokI 379 CATCC GGATG(9/13)
157 GGATG
HaeIII 225 GGCC GG^CC
526 GGCC
HapII 200 CCGG C^CGG
426 CCGG
HinfI 232 GATTC G^A[ACGT]TC
418 GAATC
HpaII 200 CCGG C^CGG
426 CCGG
HphI 512 TCACC GGTGA(8/7)
601 TCACC
MaeI 19 CTAG C^TAG
622 CTAG
MspI 200 CCGG C^CGG
426 CCGG
MwoI 130 GCTGCTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
350 GCGAGACAGGC
NciI 200 CCGGG CC^[GC]GG
426 CCGGG
NlaIII 127 CATG CATG^
250 CATG
RsaI 207 GTAC GT^AC
646 GTAC
TfiI 232 GATTC G^A[AT]TC
418 GAATC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwI Asp700I BbvI BmyI
BsiYI BslI Bsp1286I BssKI BstNI
BstYI DdeI DpnII EcoRII Fnu4HI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI NdeII
NlaIV Sau3AI ScrFI Tru9I TseI
Tsp45I Tsp509I XhoII XmnI