Restriction map for
pF1KE0683
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbsI BbvCI
BcgI BclI BfrI BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MroI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tru9I Tsp45I
Tsp509I Tth111I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 753 CCGC CCGC(-3/-1)
AlwNI 313 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 604 CCTGAGG CC^T[ACGT]AGG
ApaI 101 GGGCCC GGGCC^C
AspHI 770 GAGCTC G[AT]GC[AT]^C
BalI 247 TGGCCA TGG^CCA
BbrPI 757 CACGTG CAC^GTG
BciVI 438 GGATAC GTATCC(6/5)
BglI 240 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 757 CACGTG [CT]AC^GT[GA]
BseRI 460 GAGGAG GAGGAG(10/8)
BsgI 190 CTGCAC GTGCAG(16/14)
BsiHKAI 770 GAGCTC G[AT]GC[AT]^C
BsmAI 620 GTCTC GTCTC(1/5)
BsmBI 619 CGTCTC CGTCTC(1/5)
Bsp1407I 498 TGTACA T^GTACA
BsrGI 498 TGTACA T^GTACA
BssSI 334 CTCGTG CACGAG(-5/-1)
Bsu36I 604 CCTGAGG CC^T[ACGT]AGG
EaeI 247 TGGCCA [CT]^GGCC[GA]
Eco81I 604 CCTGAGG CC^T[ACGT]AGG
EcoT14I 416 CCAAGG C^C[AT][AT]GG
EcoT22I 676 ATGCAT ATGCA^T
FauI 752 CCCGC CCCGC(4/6)
HinfI 199 GAATC G^A[ACGT]TC
HphI 811 GGTGA GGTGA(8/7)
MaeII 758 ACGT A^CGT
MluNI 247 TGGCCA TGG^CCA
MscI 247 TGGCCA TGG^CCA
NsiI 676 ATGCAT ATGCA^T
PflMI 660 CCATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 757 CACGTG CAC^GTG
PmlI 757 CACGTG CAC^GTG
PshAI 739 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 558 CTGCAG CTGCA^G
SacI 770 GAGCTC GAGCT^C
SapI 114 GAAGAGC GCTCTTC(1/4)
ScaI 219 AGTACT AGT^ACT
SmaI 53 CCCGGG CCC^GGG
SspBI 498 TGTACA T^GTACA
SspI 700 AATATT AAT^ATT
StuI 330 AGGCCT AGG^CCT
StyI 416 CCAAGG C^C[AT][AT]GG
TaqI 681 TCGA T^CGA
TfiI 199 GAATC G^A[AT]TC
TthHB8I 681 TCGA T^CGA
Van91I 660 CCATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 708 TCTAGA T^CTAGA
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 53 CCCGGG C^[CT]CG[GA]G
104 CCCGAG
AvaII 94 GGTCC G^G[AT]CC
426 GGTCC
BanII 101 GGGCCC G[GA]GC[CT]^C
770 GAGCTC
BfaI 373 CTAG C^TAG
709 CTAG
BglII 150 AGATCT A^GATCT
567 AGATCT
BsoBI 53 CCCGGG C^[CT]CG[GA]G
104 CCCGAG
BstYI 150 AGATCT [GA]^GATC[CT]
567 AGATCT
Cac8I 210 GCCAGC GC[ACGT]^[ACGT]GC
518 GCTGGC
DdeI 257 CTCAG C^T[ACGT]AG
605 CTGAG
DraII 93 GGGTCCC [GA]G^G[ACGT]CC[CT]
425 AGGTCCT
EarI 797 CTCTTC CTCTTC(1/4)
114 GAAGAG
EcoO109I 93 GGGTCCC [GA]G^G[ACGT]CC[CT]
425 AGGTCCT
HapII 54 CCGG C^CGG
90 CCGG
HpaII 54 CCGG C^CGG
90 CCGG
Ksp632I 797 CTCTTC CTCTTC(1/4)
114 GAAGAG
MaeI 373 CTAG C^TAG
709 CTAG
MflI 150 AGATCT [GA]^GATC[CT]
567 AGATCT
MslI 51 CACCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
679 CATCGAGATG
MspA1I 316 CAGCTG C[AC]G^C[GT]G
516 CAGCTG
MspI 54 CCGG C^CGG
90 CCGG
PpuMI 93 GGGTCCC [GA]G^G[AT]CC[CT]
425 AGGTCCT
PvuII 316 CAGCTG CAG^CTG
516 CAGCTG
SfcI 430 CTACAG C^T[GA][CT]AG
558 CTGCAG
SmlI 364 CTCAAG C^T[CT][GA]AG
773 CTCAAG
XhoII 150 AGATCT [GA]^GATC[CT]
567 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI BanI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeIII ItaI MboI MboII MnlI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TseI TspRI