Restriction map for
pF1KE0674
##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT14I EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
Hin1I HpaI ItaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1073 GTAGAC GT^[AC][GT]AC
AfaI 171 GTAC GT^AC
AlwI 980 GATCC GGATC(4/5)
BciVI 1056 GTATCC GTATCC(6/5)
BmyI 394 GGGCAC G[AGT]GC[ACT]^C
BsaAI 1053 CACGTA [CT]AC^GT[GA]
BsaWI 505 ACCGGA [AT]^CCGG[AT]
BseRI 52 GAGGAG GAGGAG(10/8)
Bsp1286I 394 GGGCAC G[AGT]GC[ACT]^C
BsrDI 783 GCAATG GCAATG(2/0)
BstBI 566 TTCGAA TT^CGAA
BstYI 979 AGATCC [GA]^GATC[CT]
CfoI 1168 GCGC GCG^C
DpnII 980 GATC ^GATC
EcoRV 467 GATATC GAT^ATC
HaeIII 18 GGCC GG^CC
HapII 506 CCGG C^CGG
HgaI 572 GACGC GACGC(5/10)
HhaI 1168 GCGC GCG^C
HinP1I 1168 GCGC G^CGC
HincII 443 GTCAAC GT[CT]^[GA]AC
HindII 443 GTCAAC GT[CT]^[GA]AC
HindIII 299 AAGCTT A^AGCTT
HpaII 506 CCGG C^CGG
MaeII 1054 ACGT A^CGT
MboI 980 GATC ^GATC
MflI 979 AGATCC [GA]^GATC[CT]
MspI 506 CCGG C^CGG
NdeI 854 CATATG CA^TATG
NdeII 980 GATC ^GATC
NspV 566 TTCGAA TT^CGAA
RsaI 171 GTAC GT^AC
Sau3AI 980 GATC ^GATC
SfaNI 102 GATGC GCATC(5/9)
SfuI 566 TTCGAA TT^CGAA
TfiI 498 GAATC G^A[AT]TC
Tsp509I 225 AATT ^AATT
XhoII 979 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AluI 300 AGCT AG^CT
627 AGCT
Asp700I 456 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
792 GAAAGCATTC
BbsI 232 GAAGAC GAAGAC(2/6)
569 GAAGAC
BpuAI 232 GAAGAC GAAGAC(2/6)
569 GAAGAC
BsmAI 200 GTCTC GTCTC(1/5)
638 GTCTC
BstF5I 27 GGATG GGATG(2/0)
594 GGATG
DraII 8 AGGACCC [GA]G^G[ACGT]CC[CT]
1084 AGGACCC
EcoO109I 8 AGGACCC [GA]G^G[ACGT]CC[CT]
1084 AGGACCC
FokI 27 GGATG GGATG(9/13)
594 GGATG
PleI 198 GAGTC GAGTC(4/5)
1002 GAGTC
PpuMI 8 AGGACCC [GA]G^G[AT]CC[CT]
1084 AGGACCC
SfcI 90 CTACAG C^T[GA][CT]AG
131 CTATAG
TaqI 567 TCGA T^CGA
735 TCGA
Tsp45I 386 GTGAC ^GT[GC]AC
1004 GTCAC
TthHB8I 567 TCGA T^CGA
735 TCGA
XmnI 456 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
792 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AvaII BpmI BsaJI BsiYI BslI
BsmI BsrI BssKI BstNI Cfr13I
DdeI EcoRII HinfI HphI MaeIII
MboII MnlI MseI MslI MvaI
NlaIII NlaIV Sau96I ScrFI Tru9I
TspRI