Restriction map for
pF1KE0672
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseRI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MaeII MamI MfeI
MluI MseI MslI MspA1I MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 623 GGTACC G^GTACC
AccI 40 GTCTAC GT^[AC][GT]AC
AfaI 624 GTAC GT^AC
Alw44I 397 GTGCAC G^TGCAC
AlwNI 667 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 672 CCTGAGG CC^T[ACGT]AGG
ApaI 587 GGGCCC GGGCC^C
ApaLI 397 GTGCAC G^TGCAC
Asp700I 768 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 623 GGTACC G^GTACC
AvaI 66 CTCGGG C^[CT]CG[GA]G
BbeI 364 GGCGCC GGCGC^C
BfaI 505 CTAG C^TAG
BglI 992 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 379 CTGCAC GTGCAG(16/14)
BsmBI 369 CGTCTC CGTCTC(1/5)
BsoBI 66 CTCGGG C^[CT]CG[GA]G
BsrBI 100 GAGCGG CCGCTC(-3/-3)
Bsu36I 672 CCTGAGG CC^T[ACGT]AGG
DraIII 115 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 266 CCATGG C^C[GA][CT]GG
Eco81I 672 CCTGAGG CC^T[ACGT]AGG
EcoNI 824 CCTTCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 364 GGCGCC [GA]GCGC^[CT]
KasI 364 GGCGCC G^GCGCC
KpnI 623 GGTACC GGTAC^C
MaeI 505 CTAG C^TAG
NarI 364 GGCGCC GG^CGCC
NcoI 266 CCATGG C^CATGG
NspI 500 ACATGC [GA]CATG^[CT]
PflMI 342 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 624 GTAC GT^AC
SapI 657 GCTCTTC GCTCTTC(1/4)
SfoI 364 GGCGCC GGC^GCC
TfiI 721 GAATC G^A[AT]TC
Van91I 342 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 338 CCAGCCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 768 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 52 CGCG CG^CG
313 CGCG
AccIII 863 TCCGGA T^CCGGA
929 TCCGGA
AcyI 364 GGCGCC G[GA]^CG[CT]C
595 GGCGTC
BalI 263 TGGCCA TGG^CCA
1011 TGGCCA
BcnI 153 CCGGG CC^[GC]GG
402 CCGGG
BpmI 226 CTGGAG CTGGAG(16/14)
751 CTGGAG
BsaHI 364 GGCGCC G[GA]^CG[CT]C
595 GGCGTC
BsaI 758 GAGACC GGTCTC(1/5)
462 GGTCTC
BsaWI 863 TCCGGA [AT]^CCGG[AT]
929 TCCGGA
BseAI 863 TCCGGA T^CCGGA
929 TCCGGA
BspEI 863 TCCGGA T^CCGGA
929 TCCGGA
BspMI 377 ACCTGC ACCTGC(4/8)
778 ACCTGC
BsrI 273 ACTGG ACTGG(1/-1)
887 ACTGG
BssSI 238 CACGAG CACGAG(-5/-1)
118 CTCGTG
BstUI 52 CGCG CG^CG
313 CGCG
BstXI 266 CCATGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
338 CCAGCCATCTGG
EaeI 263 TGGCCA [CT]^GGCC[GA]
1011 TGGCCA
EarI 658 CTCTTC CTCTTC(1/4)
768 GAAGAG
EcoT14I 266 CCATGG C^C[AT][AT]GG
360 CCTTGG
FauI 983 CCCGC CCCGC(4/6)
102 GCGGG
HgaI 38 GCGTC GACGC(5/10)
596 GCGTC
Hin1I 364 GGCGCC G[GA]^CG[CT]C
595 GGCGTC
Ksp632I 658 CTCTTC CTCTTC(1/4)
768 GAAGAG
MaeIII 206 GTCAC ^GT[ACGT]AC
277 GTGAC
MluNI 263 TGGCCA TGG^CCA
1011 TGGCCA
MroI 863 TCCGGA T^CCGGA
929 TCCGGA
MscI 263 TGGCCA TGG^CCA
1011 TGGCCA
MvnI 52 CGCG CG^CG
313 CGCG
NciI 153 CCGGG CC^[GC]GG
402 CCGGG
SacI 544 GAGCTC GAGCT^C
655 GAGCTC
SmlI 127 CTCAAG C^T[CT][GA]AG
409 CTCAAG
StuI 788 AGGCCT AGG^CCT
999 AGGCCT
StyI 266 CCATGG C^C[AT][AT]GG
360 CCTTGG
TaqI 451 TCGA T^CGA
827 TCGA
Tsp45I 206 GTCAC ^GT[GC]AC
277 GTGAC
TthHB8I 451 TCGA T^CGA
827 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BanII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MflI MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI XhoII