Restriction map for
pF1KE0671
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BspDI BspHI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI Ksp632I
KspI MamI MfeI MluI MluNI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tru9I Tsp509I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 145 GGTACC G^GTACC
AccIII 459 TCCGGA T^CCGGA
AlwNI 605 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 559 CCTAAGG CC^T[ACGT]AGG
ApaI 68 GGGCCC GGGCC^C
Asp718I 145 GGTACC G^GTACC
AspI 20 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 127 CTCGAG C^[CT]CG[GA]G
BanI 145 GGTACC G^G[CT][GA]CC
BanII 68 GGGCCC G[GA]GC[CT]^C
BfaI 575 CTAG C^TAG
BglI 265 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 405 CTCCAG CTGGAG(16/14)
BsaAI 317 TACGTG [CT]AC^GT[GA]
BsaWI 459 TCCGGA [AT]^CCGG[AT]
BseAI 459 TCCGGA T^CCGGA
BsoBI 127 CTCGAG C^[CT]CG[GA]G
Bsp1407I 615 TGTACA T^GTACA
BspEI 459 TCCGGA T^CCGGA
BspMI 284 ACCTGC ACCTGC(4/8)
BsrBI 43 GAGCGG CCGCTC(-3/-3)
BsrGI 615 TGTACA T^GTACA
BssSI 368 CTCGTG CACGAG(-5/-1)
BstYI 195 GGATCT [GA]^GATC[CT]
Bsu36I 559 CCTAAGG CC^T[ACGT]AGG
DraIII 484 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 559 CCTAAGG CC^T[ACGT]AGG
EcoNI 126 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 659 CCCGC CCCGC(4/6)
KpnI 145 GGTACC GGTAC^C
MaeI 575 CTAG C^TAG
MboII 291 GAAGA GAAGA(8/7)
MflI 195 GGATCT [GA]^GATC[CT]
MroI 459 TCCGGA T^CCGGA
MspA1I 55 CAGCGG C[AC]G^C[GT]G
PaeR7I 127 CTCGAG C^TCGAG
PflFI 20 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 18 GGGACCT [GA]G^G[AT]CC[CT]
PstI 286 CTGCAG CTGCA^G
SfcI 286 CTGCAG C^T[GA][CT]AG
SfiI 264 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 127 CTCGAG C^T[CT][GA]AG
SspBI 615 TGTACA T^GTACA
TaqI 128 TCGA T^CGA
TfiI 235 GAATC G^A[AT]TC
Tth111I 20 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 128 TCGA T^CGA
XcmI 407 CCAGCTTCCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 127 CTCGAG C^TCGAG
XhoII 195 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 529 GATCC GGATC(4/5)
195 GGATC
BcnI 543 CCCGG CC^[GC]GG
731 CCCGG
BmyI 68 GGGCCC G[AGT]GC[ACT]^C
656 GTGCCC
BsmFI 18 GGGAC GGGAC(10/14)
75 GTCCC
Bsp1286I 68 GGGCCC G[AGT]GC[ACT]^C
656 GTGCCC
CfoI 571 GCGC GCG^C
735 GCGC
DdeI 312 CTTAG C^T[ACGT]AG
560 CTAAG
DpnII 196 GATC ^GATC
529 GATC
HhaI 571 GCGC GCG^C
735 GCGC
HinP1I 571 GCGC G^CGC
735 GCGC
MaeIII 320 GTGAC ^GT[ACGT]AC
578 GTGAC
MboI 196 GATC ^GATC
529 GATC
NciI 543 CCCGG CC^[GC]GG
731 CCCGG
NdeII 196 GATC ^GATC
529 GATC
NlaIII 99 CATG CATG^
738 CATG
PleI 403 GACTC GAGTC(4/5)
421 GACTC
Sau3AI 196 GATC ^GATC
529 GATC
Tsp45I 320 GTGAC ^GT[GC]AC
578 GTGAC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BsaJI BseRI BsgI BsiYI BslI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeII MnlI
MspI MvaI MwoI NlaIV RsaI
Sau96I ScrFI SfaNI TseI TspRI