Restriction map for
pF1KE0667
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AfaI AflII AgeI
AhdI AlwI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MboI
MfeI MflI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TthHB8I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 229 AAATTT [GA]^AATT[CT]
AcyI 698 GGCGTC G[GA]^CG[CT]C
Alw44I 622 GTGCAC G^TGCAC
ApaLI 622 GTGCAC G^TGCAC
ApoI 229 AAATTT [GA]^AATT[CT]
Asp700I 130 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 597 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 332 CCCGAG C^[CT]CG[GA]G
BalI 554 TGGCCA TGG^CCA
BbrPI 625 CACGTG CAC^GTG
BpmI 407 CTCCAG CTGGAG(16/14)
BsaAI 625 CACGTG [CT]AC^GT[GA]
BsaHI 698 GGCGTC G[GA]^CG[CT]C
BsgI 430 CTGCAC GTGCAG(16/14)
BsoBI 332 CCCGAG C^[CT]CG[GA]G
BspHI 351 TCATGA T^CATGA
BsrDI 185 GCAATG GCAATG(2/0)
BstF5I 687 CATCC GGATG(2/0)
DdeI 599 CTGAG C^T[ACGT]AG
DraII 656 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 100 GACTGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 554 TGGCCA [CT]^GGCC[GA]
EcoO109I 656 AGGACCC [GA]G^G[ACGT]CC[CT]
FokI 687 CATCC GGATG(9/13)
HgaI 699 GCGTC GACGC(5/10)
Hin1I 698 GGCGTC G[GA]^CG[CT]C
HphI 192 TCACC GGTGA(8/7)
MluNI 554 TGGCCA TGG^CCA
MscI 554 TGGCCA TGG^CCA
MslI 21 CATCGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 597 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 487 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 625 CACGTG CAC^GTG
PmlI 625 CACGTG CAC^GTG
PpuMI 656 AGGACCC [GA]G^G[AT]CC[CT]
PstI 326 CTGCAG CTGCA^G
RcaI 351 TCATGA T^CATGA
SacI 583 GAGCTC GAGCT^C
SfaNI 514 GATGC GCATC(5/9)
SfcI 326 CTGCAG C^T[GA][CT]AG
TfiI 10 GAATC G^A[AT]TC
Tth111I 597 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 487 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 487 CCACCTGTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 130 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwNI 96 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
593 CAGGGACTG
AspHI 583 GAGCTC G[AT]GC[AT]^C
622 GTGCAC
AvaII 565 GGACC G^G[AT]CC
657 GGACC
BanII 33 GAGCCC G[GA]GC[CT]^C
583 GAGCTC
BfaI 338 CTAG C^TAG
562 CTAG
BsaJI 36 CCCAGG C^C[ACGT][ACGT]GG
37 CCAGGG
BseRI 541 CTCCTC GAGGAG(10/8)
30 GAGGAG
BsiHKAI 583 GAGCTC G[AT]GC[AT]^C
622 GTGCAC
BsmAI 412 GAGAC GTCTC(1/5)
422 GAGAC
Cfr13I 565 GGACC G^G[ACGT]CC
657 GGACC
EarI 214 GAAGAG CTCTTC(1/4)
652 GAAGAG
HaeIII 555 GGCC GG^CC
706 GGCC
Ksp632I 214 GAAGAG CTCTTC(1/4)
652 GAAGAG
MaeI 338 CTAG C^TAG
562 CTAG
MseI 281 TTAA T^TAA
379 TTAA
NlaIII 352 CATG CATG^
589 CATG
PleI 405 GACTC GAGTC(4/5)
601 GAGTC
Sau96I 565 GGACC G^G[ACGT]CC
657 GGACC
Tru9I 281 TTAA T^TAA
379 TTAA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AflIII AluI BbvI BmyI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I EcoRII Fnu4HI HinfI
ItaI MaeII MaeIII MboII MnlI
MvaI MwoI NlaIV ScrFI TseI
Tsp45I Tsp509I TspRI