Restriction map for
pF1KE0665
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I Cfr13I
ClaI CpoI DdeI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FbaI FokI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TspRI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AfaI 339 GTAC GT^AC
AflIII 335 ACGTGT A^C[GA][CT]GT
AspHI 96 GTGCTC G[AT]GC[AT]^C
AspI 40 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 596 CCCGGG C^[CT]CG[GA]G
BanI 28 GGCACC G^G[CT][GA]CC
BfaI 617 CTAG C^TAG
BglII 706 AGATCT A^GATCT
BmyI 96 GTGCTC G[AGT]GC[ACT]^C
BsiEI 688 CGGTCG CG[GA][CT]^CG
BsiHKAI 96 GTGCTC G[AT]GC[AT]^C
BsmI 484 GAATGC GAATGC(1/-1)
BsoBI 596 CCCGGG C^[CT]CG[GA]G
Bsp1286I 96 GTGCTC G[AGT]GC[ACT]^C
BsrDI 135 CATTGC GCAATG(2/0)
BsrI 177 CCAGT ACTGG(1/-1)
BssSI 491 CACGAG CACGAG(-5/-1)
Cac8I 457 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 195 GCGC GCG^C
DraIII 322 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 624 CCGTGG C^C[GA][CT]GG
EaeI 558 TGGCCG [CT]^GGCC[GA]
EarI 171 CTCTTC CTCTTC(1/4)
EcoT22I 514 ATGCAT ATGCA^T
FauI 546 GCGGG CCCGC(4/6)
HapII 597 CCGG C^CGG
HgaI 603 GCGTC GACGC(5/10)
HhaI 195 GCGC GCG^C
HinP1I 195 GCGC G^CGC
HincII 46 GTCAAC GT[CT]^[GA]AC
HindII 46 GTCAAC GT[CT]^[GA]AC
HinfI 716 GATTC G^A[ACGT]TC
HpaII 597 CCGG C^CGG
HphI 433 TCACC GGTGA(8/7)
Ksp632I 171 CTCTTC CTCTTC(1/4)
MaeI 617 CTAG C^TAG
MseI 404 TTAA T^TAA
MslI 229 CACTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 597 CCGG C^CGG
NlaIV 28 GGCACC GG[ACGT]^[ACGT]CC
NsiI 514 ATGCAT ATGCA^T
NspI 76 GCATGT [GA]CATG^[CT]
PflFI 40 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 321 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 622 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 339 GTAC GT^AC
SexAI 323 ACCTGGT A^CC[AT]GGT
SmaI 596 CCCGGG CCC^GGG
TaqI 691 TCGA T^CGA
TfiI 716 GATTC G^A[AT]TC
Tru9I 404 TTAA T^TAA
Tsp509I 69 AATT ^AATT
Tth111I 40 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 691 TCGA T^CGA
Van91I 321 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 616 TCTAGA T^CTAGA
XmaCI 596 CCCGGG C^CCGGG
XmaI 596 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbvI 461 GCTGC GCAGC(8/12)
540 GCTGC
BcnI 596 CCCGG CC^[GC]GG
597 CCGGG
BsaBI 581 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
587 GATCCCGATC
BsaI 317 GAGACC GGTCTC(1/5)
620 GAGACC
BstNI 312 CCTGG CC^[AT]GG
324 CCTGG
BstYI 586 AGATCC [GA]^GATC[CT]
706 AGATCT
EcoRII 312 CCTGG ^CC[AT]GG
324 CCTGG
HaeIII 15 GGCC GG^CC
559 GGCC
MamI 581 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
587 GATCCCGATC
MflI 586 AGATCC [GA]^GATC[CT]
706 AGATCT
MvaI 312 CCTGG CC^[AT]GG
324 CCTGG
NciI 596 CCCGG CC^[GC]GG
597 CCGGG
SfaNI 219 GATGC GCATC(5/9)
364 GATGC
TseI 461 GCTGC G^C[AT]GC
540 GCTGC
XhoII 586 AGATCC [GA]^GATC[CT]
706 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI AlwI BsaJI BseRI
BsiYI BslI BsmAI BssKI DpnII
Fnu4HI ItaI MaeII MaeIII MboI
MboII MnlI MwoI NdeII NlaIII
Sau3AI ScrFI Tsp45I