Restriction map for
pF1KE0657
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspMI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DpnII DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FbaI FokI FseI FspI
HaeII HapII HhaI HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MamI
MboI MflI MluI MluNI MroI
MscI MslI MspI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NspV
PacI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Van91I
XbaI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 64 CCGC CCGC(-3/-1)
AcyI 620 GGCGTC G[GA]^CG[CT]C
AflIII 692 ACATGT A^C[GA][CT]GT
Alw44I 139 GTGCAC G^TGCAC
ApaLI 139 GTGCAC G^TGCAC
AspHI 139 GTGCAC G[AT]GC[AT]^C
AspI 538 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 317 CTCGAG C^[CT]CG[GA]G
AvaII 57 GGTCC G^G[AT]CC
BcgI 246 CGAATTCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 296 CTAG C^TAG
BmyI 139 GTGCAC G[AGT]GC[ACT]^C
BsaHI 620 GGCGTC G[GA]^CG[CT]C
BsaI 455 GAGACC GGTCTC(1/5)
BseRI 452 GAGGAG GAGGAG(10/8)
BsiHKAI 139 GTGCAC G[AT]GC[AT]^C
BsmAI 455 GAGAC GTCTC(1/5)
BsmFI 242 GTCCC GGGAC(10/14)
BsoBI 317 CTCGAG C^[CT]CG[GA]G
Bsp1286I 139 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 184 TGTACA T^GTACA
BspLU11I 692 ACATGT A^CATGT
BsrBI 64 CCGCTC CCGCTC(-3/-3)
BsrGI 184 TGTACA T^GTACA
Cac8I 592 GCTCGC GC[ACGT]^[ACGT]GC
Cfr13I 57 GGTCC G^G[ACGT]CC
DsaI 29 CCACGG C^C[GA][CT]GG
EcoRI 247 GAATTC G^AATTC
EcoT14I 351 CCTTGG C^C[AT][AT]GG
EcoT22I 214 ATGCAT ATGCA^T
Hin1I 620 GGCGTC G[GA]^CG[CT]C
HinfI 121 GAATC G^A[ACGT]TC
HphI 68 TCACC GGTGA(8/7)
MaeI 296 CTAG C^TAG
MaeIII 16 GTCAC ^GT[ACGT]AC
MboII 580 GAAGA GAAGA(8/7)
MspA1I 460 CAGCTG C[AC]G^C[GT]G
NlaIV 24 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 214 ATGCAT ATGCA^T
NspI 692 ACATGT [GA]CATG^[CT]
PaeR7I 317 CTCGAG C^TCGAG
PflFI 538 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 51 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 460 CAGCTG CAG^CTG
Sau96I 57 GGTCC G^G[ACGT]CC
SfcI 115 CTACAG C^T[GA][CT]AG
SspBI 184 TGTACA T^GTACA
StyI 351 CCTTGG C^C[AT][AT]GG
TfiI 121 GAATC G^A[AT]TC
Tsp45I 16 GTCAC ^GT[GC]AC
Tth111I 538 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 29 CCACGGCAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 317 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 247 GAATTC [GA]^AATT[CT]
384 AAATTC
AfaI 133 GTAC GT^AC
185 GTAC
ApoI 247 GAATTC [GA]^AATT[CT]
384 AAATTC
BpmI 125 CTGGAG CTGGAG(16/14)
416 CTGGAG
BsiYI 252 CCGTGCGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
617 CCAGGCGTCGG
BslI 252 CCGTGCGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
617 CCAGGCGTCGG
BsrI 105 ACTGG ACTGG(1/-1)
136 CCAGT
MfeI 95 CAATTG C^AATTG
637 CAATTG
MseI 233 TTAA T^TAA
359 TTAA
MunI 95 CAATTG C^AATTG
637 CAATTG
RsaI 133 GTAC GT^AC
185 GTAC
SmlI 317 CTCGAG C^T[CT][GA]AG
523 CTCAAG
StuI 348 AGGCCT AGG^CCT
441 AGGCCT
TaqI 195 TCGA T^CGA
318 TCGA
Tru9I 233 TTAA T^TAA
359 TTAA
TthHB8I 195 TCGA T^CGA
318 TCGA
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BbvI BsaJI BssKI BstAPI
BstNI EcoRII Fnu4HI HaeIII HgaI
ItaI MnlI MvaI MwoI NlaIII
ScrFI TseI Tsp509I TspRI