Restriction map for
pF1KE0656
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AvaI AviII BamHI
BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrI BglI BmyI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1286I BspEI BsrDI BsrFI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CfoI Cfr10I CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 480 CGCG CG^CG
Asp700I 973 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 523 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 333 CCTAGG C^CTAGG
BalI 749 TGGCCA TGG^CCA
BbrPI 33 CACGTG CAC^GTG
BbvI 397 GCAGC GCAGC(8/12)
BclI 264 TGATCA T^GATCA
BcnI 243 CCCGG CC^[GC]GG
BfaI 334 CTAG C^TAG
BglII 142 AGATCT A^GATCT
BlnI 333 CCTAGG C^CTAGG
BlpI 718 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 718 GCTAAGC GC^T[ACGT]AGC
BsaAI 33 CACGTG [CT]AC^GT[GA]
BsgI 78 GTGCAG GTGCAG(16/14)
Bsp1407I 662 TGTACA T^GTACA
BspDI 577 ATCGAT AT^CGAT
BspLU11I 854 ACATGT A^CATGT
BspMI 953 GCAGGT ACCTGC(4/8)
BsrBI 293 GAGCGG CCGCTC(-3/-3)
BsrGI 662 TGTACA T^GTACA
BstUI 480 CGCG CG^CG
BstYI 142 AGATCT [GA]^GATC[CT]
CelII 718 GCTAAGC GC^T[ACGT]AGC
ClaI 577 ATCGAT AT^CGAT
EcoRI 25 GAATTC G^AATTC
EcoT22I 652 ATGCAT ATGCA^T
FbaI 264 TGATCA T^GATCA
Fnu4HI 397 GCAGC GC^[ACGT]GC
HindIII 1018 AAGCTT A^AGCTT
ItaI 397 GCAGC GC^[ACGT]GC
MaeI 334 CTAG C^TAG
MaeII 34 ACGT A^CGT
MflI 142 AGATCT [GA]^GATC[CT]
MluNI 749 TGGCCA TGG^CCA
MscI 749 TGGCCA TGG^CCA
MvnI 480 CGCG CG^CG
NciI 243 CCCGG CC^[GC]GG
NsiI 652 ATGCAT ATGCA^T
PflFI 523 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 33 CACGTG CAC^GTG
PmlI 33 CACGTG CAC^GTG
PstI 951 CTGCAG CTGCA^G
SbfI 950 CCTGCAGG CCTGCA^GG
SfcI 951 CTGCAG C^T[GA][CT]AG
SmlI 1066 CTCAAG C^T[CT][GA]AG
Sse8387I 950 CCTGCAGG CCTGCA^GG
SspBI 662 TGTACA T^GTACA
TseI 397 GCAGC G^C[AT]GC
Tth111I 523 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 142 AGATCT [GA]^GATC[CT]
XmnI 973 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 498 GTAC GT^AC
663 GTAC
BpmI 1101 CTCCAG CTGGAG(16/14)
55 CTGGAG
BsmAI 373 GAGAC GTCTC(1/5)
928 GAGAC
BsmBI 373 GAGACG CGTCTC(1/5)
928 GAGACG
BsmFI 128 GGGAC GGGAC(10/14)
959 GGGAC
BspHI 461 TCATGA T^CATGA
620 TCATGA
EcoT14I 333 CCTAGG C^C[AT][AT]GG
1001 CCAAGG
HapII 244 CCGG C^CGG
253 CCGG
HgaI 930 GACGC GACGC(5/10)
458 GCGTC
HincII 235 GTTGAC GT[CT]^[GA]AC
298 GTCAAC
HindII 235 GTTGAC GT[CT]^[GA]AC
298 GTCAAC
HpaII 244 CCGG C^CGG
253 CCGG
MseI 4 TTAA T^TAA
1028 TTAA
MslI 361 CACAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
849 CACTGACATG
MspI 244 CCGG C^CGG
253 CCGG
MwoI 247 GCTCCTCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1054 GCAAGCGAAGC
NspI 650 ACATGC [GA]CATG^[CT]
854 ACATGT
PleI 1106 GACTC GAGTC(4/5)
511 GAGTC
RcaI 461 TCATGA T^CATGA
620 TCATGA
RsaI 498 GTAC GT^AC
663 GTAC
StyI 333 CCTAGG C^C[AT][AT]GG
1001 CCAAGG
TaqI 509 TCGA T^CGA
578 TCGA
Tru9I 4 TTAA T^TAA
1028 TTAA
TspRI 76 CAGTG CA[GC]TG[ACGT][ACGT]^
849 CACTG
TthHB8I 509 TCGA T^CGA
578 TCGA
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcsI AflIII AluI ApoI
AvaII BsaJI BseRI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
FokI HaeIII HinfI HphI Ksp632I
MaeIII MboI MboII MnlI MspA1I
MvaI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI TfiI
Tsp45I Tsp509I